Summary
| Production Name | Flag-tag (M11) Rabbit Monoclonal Antibody |
| Application | WB,ELISA,IF |
| Reactivity | Species independent |
| Purification | Protein A purified |
| Gene Name | Flag |
| Immunogen | Synthetic Peptide of Flag-Tag |
| Specificity | The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins. |
| Dilution Ratio | WB 1:1000-5000, ELISA 1:20000-50000, IF-ICC 1:100-300 |
| Host | Rabbit |
| Isotype | IgG |
| Buffer | PBS, pH 7.4, containing 0.5%protective proteinand 50% Glycerol. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. |
