Recombinant Human TFIIB (N-GST)

Recombinant Human TFIIB (N-GST)

Size1:10μg price1:$168
Size2:50μg price2:$465
Size3:500μg price3:$2350 SKU: PEH1707 Category: Target Proteins Tags: ,

Datasheet

Name

Recombinant Human TFIIB (N-GST)

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin level

<1 EU/µg as determined by LAL test.

Construction

Recombinant Human Transcription Initiation Factor IIB is produced by our E.coli expression system and the target gene encoding Met1-Leu316 is expressed with a GST tag at the N-terminus.

Accession #

Q00403

Host

E.coli

Species

Human

Predicted Molecular Mass

61.64 KDa

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.

Form

Lyophilized

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Stability&Storage

Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

 

 

Alternative Names

Transcription Initiation Factor IIB; General Transcription Factor TFIIB; S300-II; GTF2B; TF2B; TFIIB

 

Background

Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.

 

Note

For Research Use Only , Not for Diagnostic Use.

 

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