Custom Antibody Services
Antibodies are generated when B cells, upon antigenic stimulation, differentiate into plasma cells. Polyclonal preparations:produced rapidly and economically,recognize multiple epitopes;whereas monoclonal antibodies, derived from a single B-cell clone, exhibit exceptional specificity and uniformity, making them the reagents of choice for therapeutic, diagnostic, and research applications.EnkiLife provides polyclonal & monoclonal antibody production with high specificity and purity. From antigen design to affinity purification, we deliver ready-to-use antibodies validated for ELISA, WB, IHC, and SPR.
Explore Services
Service Portfolio
| Service Module | Description | Timeline |
|---|---|---|
| Polyclonal Package | ||
| Antigen Preparation | Customer or EnkiLife supplied | 4-6 weeks |
| Immunization & Bleed | New Zealand rabbit, ELISA-driven bleeds | 6-8 weeks |
| Affinity Purification | Antigen-column, QC by SDS-PAGE & ELISA | 1-2 weeks |
| Mouse Monoclonal Package | ||
| Antigen Preparation | Customer or EnkiLife supplied | 4-6 weeks |
| Immunization & Titer | BalB/C mice, ELISA screening | 6-8 weeks |
| Cell Fusion & Screening | Hybridoma generation, HAT selection | 2 weeks |
| Sub-cloning | Single-cell dilution, expansion | 4-5 weeks |
| Ascites / Supernatant | Mouse ascites or roller-bottle production | 2-3 weeks |
| Antibody Purification | Protein A/G, endotoxin removal | 1-2 weeks |
| Rabbit Monoclonal Package | ||
| B-cell Sorting | Flow-cytometry single-B-cell isolation | 1 week |
| Sequencing & Cloning | NGS of VH/VL, mammalian expression vector | 1 week |
| Recombinant Expression | HEK293/CHO transient, endotoxin-free | 2-4 weeks |
| Optional Add-ons | HRP/FITC/Biotin labeling, pairing, sequencing | On request |
Deliverables
★ Purified antibody (≥ 95 %)
★ Hybridoma cell line or sequence report
★ QC dossier (SDS-PAGE, ELISA, endotoxin)
Case Study
Produced recombinant rabbit monoclonal antibody validated in WB, IF, IHC.
 |  |  |  |  |  | |
| SDS-PAGE with purified antibody | WB | IF | IHC results with human liver cancer | IHC results with placenta |
Contact Us
To discuss your custom antibody needs, please email order@enkilife.com, our team is ready to assist you right away.
Download Custom Antibody Request Form