Custom Antibody Services
EnkiLife provides end‑to‑end custom antibody development — from immunogen design, animal immunization (rabbit, mouse, rat, chicken) to hybridoma, phage display, recombinant expression, and antibody sequencing/engineering. High affinity, specific, and reproducible antibodies for research, diagnostic and therapeutic applications.
Explore Platforms
High‑quality antibodies drive reproducible science
Antibodies are essential tools for detecting, quantifying, and modulating targets. The success of your assay (WB, IHC, IP, FACS, IF) depends on antibody specificity and affinity. EnkiLife’s custom antibody platform integrates advanced antigen design, multiple host species, and rigorous screening to deliver antibodies that work the first time.
We offer polyclonal, monoclonal (hybridoma), recombinant antibody production, as well as phage display library generation, antibody sequencing, and humanization/affinity maturation. Whether you need a few milligrams for ELISA or gram-scale recombinant antibodies, our platform scales accordingly.
Antibody discovery & production platforms
Polyclonal Antibody
Monoclonal Antibody
Recombinant
Phage display
Sequencing & engineering
Platform comparison at a glance
Detailed side‑by‑side comparison of antibody platforms — from polyclonal to phage display and engineering.
| Feature / Platform | Polyclonal | Monoclonal (hybridoma) | Recombinant | Phage display | Sequencing & engineering |
|---|---|---|---|---|---|
| Specificity | Multiple epitopes (polyclonal mixture) higher avidity, but batch variability | Single defined epitope high specificity; rare clone drift | Single defined epitope sequence-defined, no drift | Single defined epitope selected by panning, very stringent | Depends on parental Ab; can be refined humanization, affinity maturation |
| Affinity | High overall (avidity effect) | Clone-dependent (can be very high) | Defined by sequence; engineerable to pM | Can be matured to pM via library selection | Enables affinity maturation (in vitro) |
| Batch consistency | Poor (animal-to-animal variation) | High (same hybridoma line) | Very high (defined DNA sequence) | Very high (once sequence is fixed) | N/A (service output is sequence/engineered gene) |
| Development time | 2–3 months | 4–6 months | 2–6 weeks (if sequence known) or 4–5 months (de novo) | 3–4 months (library + panning) | 1–3 months (depending on complexity) |
| Cost | Low | High | Medium to High | High (library construction & screening) | Variable |
| Animal use | Required (immunization & bleeding) | Required (immunization for B cells) | Optional (if synthetic or from display) | No animals (in vitro only) | No animals (in vitro) |
| Humanization potential | Not feasible | Limited (requires sequencing then recombinant) | Yes (CDR grafting, etc.) | Inherent (naïve human libraries give fully human) | Core service (humanization, deimmunization) |
| Format switching (scFv, Fab, bispecific) | Not feasible | Limited (requires sequencing) | Yes (highly flexible) | Yes (scFv/Fab directly available) | Yes (main output) |
| Fc engineering | Not feasible | Not feasible directly | Yes (point mutations, glycoengineering) | Yes (via recombinant expression after selection) | Yes (design and provide engineered genes) |
| Typical applications | IP, WB, IHC (paraffin), ELISA capture, lateral flow | FACS, IHC, IF, neutralization, diagnostic kits, therapeutic leads | Therapeutics, IVD grade reagents, consistent detection, ADC | Fully human therapeutic discovery, difficult antigens, VHH | Hybridoma rescue, humanization, affinity optimization, bispecific design |
*Recombinant antibodies can originate from hybridoma sequencing, single B cell cloning, or synthetic libraries. Phage display outputs are typically reformatted as recombinant antibodies.
Why EnkiLife for antibody development
- Multiple host species – rabbit, mouse, rat, chicken, llama – choose the best immune response.
- Integrated antigen services – peptide synthesis, protein expression (see our protein services), conjugation.
- High success rate for difficult targets (GPCRs, ion channels, conserved proteins) via advanced immunization or phage display.
- Sequencing & recombinant rescue – we can sequence your hybridoma and convert to recombinant antibody.
- Downstream engineering – humanization, affinity maturation, bispecific, Fc fusion, ADCC enhancement.
- Flexible scale – from 1 mg to 10 g purified antibody, with rigorous QC (SDS-PAGE, SEC-HPLC, endotoxin).
Start your custom antibody project
Fill out our antibody project request form or email order@enkilife.com. Our scientists will reply within 24 hours to discuss your target and recommend the optimal strategy.