Product Introduction
The EnkiLife Biotin Labeling Kit offers a selection of biotin types commonly used in laboratories. Each kit includes all necessary reagents for labeling, designed specifically for proteins and antibodies with primary amino groups. The components of this kit are intended for labeling antibodies or proteins of comparable size to antibodies, by default.
Features
lFast: Labeling takes only about 30 minutes.
lRich in variety: We provide a variety of biotin types to meet various application requirements.
lOutstanding effect: targeted optimization of labeling buffer, labeling site is far away from antigen binding site, and labeled antibody has high homogeneity.
lConvenient: Each biotin reagent has been optimized and designed with the corresponding antibody amount, and has been activated and can be used directly, without the need for tedious calculations. The batches are stable and the best results can be achieved by following the steps.
Biotin Information
Biotin Type | Spacer arm length | Solubility | Key Features | Catalog Number |
13.5 Å | Water Solubility | Membrane impermeable, short arms, sulfonic acid group, high solubility in aqueous solution | RE80002q | |
22.4 Å | Water Solubility | Membrane impermeable, medium arm length, sulfonic acid group, high solubility in aqueous solution | RE80002s | |
30.5 Å | Water Solubility | Membrane impermeable, enhanced arm length, sulfonic acid group, high solubility in aqueous solution | RE80002u | |
24.3 Å | Water Solubility | Membrane impermeable, selective cutting possible | RE80002v | |
29.0 Å | Water Solubility | PEGylation to prevent protein aggregation | RE80002w |
Selection of labeling kit
lWhen the molecular weight of the final labeled product needs to be as small as possible, choose the Short-Arm Biotin Labeling Kit.
lWhen follow-up applications require the use of reducing agents to cleave the biotin tag, choose the Sulfo-NHS-SS-Biotin Labeling Kit.
lWhen you need to maximize protein solubility and prevent protein aggregation, choose the NHS-PEG4-Biotin labeling kit.
lWhen you want the final binding steric hindrance to be smaller, choose the long-arm biotin labeling kit and select the appropriate arm length based on the binding characteristics.
Labeling principle
Within a certain pH range, biotin reacts specifically with primary amino groups to form stable amide bonds, thereby achieving coupling with proteins.
Product composition
Product composition | Content of components in different specifications | Storage temperature | ||
40 μg antibody | 200 μg antibody | 2 mg antibody | ||
Sulfo-NHS-Biotin | 1 tube | 5 tubes | 5 tubes | -20 °C after opening, away from light |
Ultrafiltration Tube * ,50K MWCO | 1 set ** | 1 set ** | 1set ** | RT |
Labeling Buffer B | 10 mL | 10 mL | 10 mL | 2-8 °C |
1× PBS (pH 7.4) | 10 mL | 10 mL | 10 mL | 2-8 °C |
DMF | 100 μL | 100 μL | 100 μL | 2-8 °C, away from light |
Labeled protein storage solution | 1 mL | 1 mL | 5 mL | 2-8°C |
Recommended amount of labeled antibody | Each tube is labeled with 20-40 μg, and the best is 20 μg | Each tube is labeled with 20-40 μg, and the best is 20 μg | Each tube is labeled with 100-400 μg, and the best is 200 μg | |
Conventional Antibody Protein Labeling Process
Step 1：Ultrafiltration buffer exchange and concentration
Step 2：Labeling reaction
Step 3：Purification
Step 4：Collection and preservation
