EnkiLife HRP labeling kit contains multiple components for labeling antibody , such as the activated lyophilized HRP, labeling buffer, and other chemical reagents, enabling easy and reliable antibody labeling.
Features
l The kit has complete components and is easy to operate. You can obtain high-quality conjugated antibodies by simply following the operating steps.
l The HRP configured in this kit is of high purity and high activity, and has a higher catalytic efficiency for substrates.
l This reagent uses directional coupling technology, and neither the HRP nor the antibody will self-couple, ensuring the specificity and uniformity of the conjugate.
l The cross-linker used in the kit has an extended arm to ensure that the application effect is not affected by protein steric hindrance.
Kit Specifications
Product composition | Content of components in different specifications | |||
80 μg antibody | 400 μg antibody | 4mg antibody | Storage temperature | |
Activated HRP | 1 tube | 5 tube | 5 tubes | -20 °C, away from light |
Labeling Buffer H | 5 mL | 5 mL | 5 mL | 4 °C, away from light |
Antibody Modification Reagents | 60 μL | 60 μL | 60 μL×2 | -20 °C, away from light |
Ultrafiltration Tube * ,50K MWCO | 1 set ** | 1 set ** | 1 set ** | RT |
Blocking reagent | 300 μg | 300 μg | 300 μg×4 | -20 °C, away from light |
DMSO (dissolve blocking reagent) | 50 μL | 50 μL | 200 μL | -20 °C, away from light |
Labeled protein storage solution | 1mL | 1mL | 5mL | 2-8 °C |
Recommended amount of labeled antibody | Each tube is labeled with 20-80μg, and the best is 20μg | Each tube is labeled with 20-80μg, and the best is 20μg | Each tube is labeled with 200-800μg, and the best is 200μg | |
Buffer constituent compatibility
Components | Kit Labeling Compatibility |
Sodium azide <0.1%, glycerol <50%, Tris <50mM, glycine <50mM, Proclin <0.5%, EDTA <10mM, trehalose <5%, sucrose <5%, or other small molecule additives | Yes, perform ultrafiltration and concentration of antibody samples, i.e. labeling step 1. |
Antibodies in ascites, serum, and cell culture supernatant | No, perform the purification step before using this kit |
Contains BSA>0.1% | No, use a BSA removal kit (such as RE80028: BSA Removal Kit) to purify the antibody before using this kit |
Antibody concentration is less than 2 mg/mL | Yes, perform ultrafiltration and concentration of antibody samples, i.e. labeling step 1. |
Antibody concentration ≥ 2 mg/mL, and does not contain BSA (< 0.1% ), gelatin (< 0.1% ), and small molecule additives. | Yes, no need for ultrafiltration concentration and buffer exchange. Directly dilute it to 1mg/mL using the Labeling Buffer H. |
Conventional Antibody Labeling Process
STEP 1：Ultrafiltration buffer exchange and concentration (optional)
STEP 2：Antibody activation
STEP 3：Labeling reaction
STEP 4：Block & Purify
STEP 5：Storage
Other Issues:
Refer to the FAQ section
