Basic Information
Product name | Mitochondrial Tracker (Green) |
Size | 50 μg/20 x 50 μg |
Storage | -20℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Mitochondrial Tracker (Green) excitation/emission wavelength: 490/523nm
Molecular Structure
Product Introduction
Mitochondrial Tracker (Green) (mitochondrial green fluorescent probe) is a mitochondrial green fluorescent dye that can stain and mark mitochondria at the nanomolar level. The dye has plasma membrane permeability and accumulates on the mitochondrial membrane to show bright fluorescence. The localization of the dye in mitochondria is independent of the mitochondrial membrane potential. The signal-to-noise ratio of stained fixed cells is not ideal. After cell fixation and permeabilization , the fluorescent signal will be weakened or lost, so we recommend that it is only used to stain live cells.
Reagent Preparation
Preparation of stock solution
Prepare 200 μM Mitochondrial Tracker (Green) stock solution: Take a 50 ug tube of Mitochondrial Tracker (Green) dye, add 372 μL of anhydrous DMSO or DMF, and vortex to mix thoroughly. This stock solution is stable at-20°C for 6 months.
The optimal dye concentration and incubation time vary with cell types. We recommend a working concentration of 20-200 nM for Mitochondrial Tracker (Green).It is easy to stain other cell structures.
Note
1. All fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching.
2. To avoid repeated freezing and thawing, this product can be repacked into small quantities.
3. This product is For Research Use Only, Not for Diagnostic Use.
