Basic Information
Product Name | Fluo-4 AM, Calcium Ion Fluorescent Probe |
Size | 50 μg |
Storage | -20℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Fluo-4 AM excitation /emission wavelength: 494 / 516nm
Molecular Structure
Product Introduction
Fluo-4 is a calcium fluorescent probe that replaces the Cl ions in the Fluo-3 structure with F ions. Since the Cl ions are replaced with F ions with stronger electron attraction, its maximum excitation wavelength will deviate by about 10 nm toward a shorter wavelength. This wavelength is closer to the wavelength of an argon laser, so when excited by an argon laser, the fluorescence intensity of Fluo-4 is stronger than that of Fluo-3.
Fluo-4, AM ester penetrates the cell membrane and enters the cell, where it is cleaved by the esterase in the cell to form Fluo-4, which is then retained in the cell. Fluo-4 is almost non-fluorescent when it exists as a free ligand, but it can produce strong fluorescence when combined with intracellular calcium ions. Laser confocal microscopy or flow cytometry can be used to detect changes in intracellular calcium ion concentrations.
Precautions
1. If a culture medium containing serum is used, the esterase in the serum will decompose the AM ester body, thereby reducing the effect of Fluo-4, AM ester entering the cells. In addition, a culture medium containing phenol red will slightly increase the background value. Before adding the working solution, the residual culture medium should be removed as much as possible.
2. All fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching.
3. Fluo-4, AM ester is easy to absorb moisture. After taking it out of the refrigerator, please make sure it is in a dry environment and put it at room temperature before opening. Since the reagent is very small, please centrifuge it briefly before opening to ensure that the powder falls to the bottom of the tube.
4. Fluo-4, AM ester is easily decomposed when in contact with water. If it cannot be used up all at once, it is recommended to store the stock solution in small portions.
Note: This reagent is for scientific research use only!
