EnkiLife APC labeling kit contains multiple components for labeling antibody proteins, such as the activated lyophilized APC fluorescent dye, labeling buffer, and other chemical reagents, enabling easy and reliable antibody labeling for use as fluorescent probes.
Features
Activated dye with extended arm: A specially designed long flexible arm minimizes spatial hindrance between antibodies and the fluorescent dye. This protects antibody activity and enhances labeling efficiency.
Site-specific conjugation: Optimized site-specific conjugation technology ensures uniform antibody labeling and improves efficiency.
Rapid process: The simple procedure allows for efficient labeling completion in as fast as 3 - 4 hours.
High dye brightness: The selected high-performance fluorescent dye boasts high quantum yield and brightness, ensuring high detection sensitivity.
Dye stability: The chosen high-performance fluorescent dye is cross-linked and stable, resistant to degradation, and suitable for various subsequent applications, such as fixed sample detection.
User-friendly operation: Each dye reagent is pre-optimized for corresponding antibody amounts. No complex calculations or ultrafiltration steps are required if the antibody sample meets the labeling conditions. The solid dye form ensures batch stability. Simply mix the reagents according to the steps to achieve good results.
Flexibility: In the large-scale kit, the dye is pre-portioned for consistent batch performance, facilitating multiple labeling uses.
Comprehensive reagents: The kit includes a labeling preservation solution, covering the entire process from labeling to preservation. No additional reagents are needed, making the process worry-free and convenient.
Dye Introduction
Allophycocyanin(APC) is a phycobiliprotein isolated from Spirulina (a type of cyanobacteria) with a molecular weight of 104 kDa and a high quantum yield. It can be excited to emit light at 594 nm and 633 nm, with a maximum absorption peak at 650 nm and a maximum fluorescence emission peak at 662 nm. In the labeling kit,the APC is cross-linked , with an extremely high absorbtivity, a high quantum efficiency and excitation andemission peaks at visible wavelengths. It can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics.
Properties of APC Dye
Form: Blue solid
Molecular Weight: ~ 104 kDa
Excitation Light Sources: 630~640 nm
Excitation Wavelengths: 650 nm
Emission Wavelength: 662 nm
Molar Extinction Coefficient (L/(mol・cm)): 700000
Purity: A650/A280 ≥ 5.0
Cross Link Ratio:≥1.2
Solubility: Highly soluble in aqueous solutions
Activating Group: Maleimide (MAL)
Activation Effect: MAL/APC= 1.2- 3
APC Spectrum
Kit Specifications
Product composition | Content of components in different specifications | |||
40 μg antibody | 200 μg antibody | 1 mg antibody | Storage temperature | |
Activated APC | 1 tube | 1 tube | 5 tubes | -20 °C, away from light |
Labeling Buffer L | 2 mL | 2 mL | 2 mL | 4 °C, away from light |
Antibody Modification Reagents | 60 μL | 60 μL | 60 μL | -20 °C, away from light |
Ultrafiltration Tube * ,50K MWCO | 1 set ** | 1 set ** | 1 set ** | RT |
Blocking reagent | 300 μg | 300 μg | 300 μg | -20 °C, away from light |
DMSO (dissolve blocking reagent) | 50 μL | 50 μL | 50 μL | -20 °C, away from light |
Labeled protein storage solution | 200 μL | 1 mL | 5 mL | 2-8 °C |
Recommended amount of labeled antibody | Each tube is labeled with 20-40 μg, and the best is 20 μg | Each tube is labeled with 50-200 μg, and the best is 100 μg | Each tube is labeled with 50-200 μg, and the best is 100 μg | |
Conventional Antibody Labeling Process
STEP 1：Ultrafiltration buffer exchange and concentration (optional)
STEP 2：Antibody activation
STEP 3：Labeling reaction
STEP 4：Quench/Blocking and preservation
Other Issues:
Refer to the FAQ section
