KEY FEATURES
| Full Name | Vascular Endothelial Growth Factor 165 |
|---|---|
| Synonym | VEGF165 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 31.25-2000pg/mL |
| Sensitivity | 18.75pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human VEGF165 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF165 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF165, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF165. You can calculate the concentration of Human VEGF165 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human VEGF165 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 91.19 | 315.08 | 759.23 | 96.66 | 294.22 | 707.86 |
| Standard deviation | 5.12 | 13.39 | 40.54 | 5.02 | 16.39 | 38.08 |
| CV(%) | 5.62 | 4.25 | 5.34 | 5.19 | 5.57 | 5.38 |
RECOVERY
The recovery of Human VEGF165 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-99 | 94 |
| EDTA plasma (n=8) | 84-98 | 90 |
| Cell culture media (n=8) | 92-104 | 99 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human VEGF165 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Pathways associated with ELISA Kit
| Alpha6-Beta4 Integrin Signaling Pathway | Alpha6-Beta4 Integrin Signaling Pathway |
| Apoptosis Pathway | Apoptosis Pathway |
| Apoptotic Cleavage Of Cellular Proteins Pathway | Apoptotic Cleavage Of Cellular Proteins Pathway |
| Apoptotic Execution Phase Pathway | Apoptotic Execution Phase Pathway |
| Aurora B Signaling Pathway | Aurora B Signaling Pathway |
| Caspase Cascade In Apoptosis Pathway | Caspase Cascade In Apoptosis Pathway |
| Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway | Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway |
| Epstein-Barr Virus Infection Pathway | Epstein-Barr Virus Infection Pathway |
| MicroRNAs In Cancer Pathway | MicroRNAs In Cancer Pathway |
Diseases associated with VIM ELISA Kit
| Nervous System Diseases | publications with VIM and Nervous System Diseases |
| Neurodegenerative Diseases | publications with VIM and Neurodegenerative Diseases |
| Inflammation | publications with VIM and Inflammation |
| Kidney Diseases | publications with VIM and Kidney Diseases |
| Pain | publications with VIM and Pain |
| Disease Models, Animal | publications with VIM and Disease Models, Animal |
| Hemorrhage | publications with VIM and Hemorrhage |
| Neoplasm Metastasis | publications with VIM and Neoplasm Metastasis |
| Liver Diseases | publications with VIM and Liver Diseases |
| Breast Neoplasms | publications with VIM and Breast Neoplasms |
Organs/Tissues associated with VIM ELISA Kit
| Brain | publications with VIM and Brain |
| Blood | publications with VIM and Blood |
| Muscle | publications with VIM and Muscle |
| Nerve | publications with VIM and Nerve |
| Bone | publications with VIM and Bone |
| Kidney | publications with VIM and Kidney |
| Liver | publications with VIM and Liver |
| Bone Marrow | publications with VIM and Bone Marrow |
| Eye | publications with VIM and Eye |
| Ganglia | publications with VIM and Ganglia |
