KEY FEATURES
| Full Name | Procollagen I |
|---|---|
| Synonym | PCOL-I; PC-I; PC1; Type I Procollagen |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human PC-Ⅰ in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PC-Ⅰ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PC-Ⅰ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PC-Ⅰ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PC-Ⅰ. You can calculate the concentration of Human PC-Ⅰ in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human PC-Ⅰ ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.54 | 1.18 | 4.1 | 0.51 | 1.13 | 4.48 |
| Standard deviation | 0.03 | 0.06 | 0.13 | 0.03 | 0.06 | 0.14 |
| CV(%) | 5.12 | 5.5 | 3.13 | 5.99 | 5.22 | 3.19 |
RECOVERY
The recovery of Human PC-Ⅰ spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-104 | 99 |
| EDTA plasma (n=8) | 87-99 | 94 |
| Cell culture media (n=8) | 84-98 | 90 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human PC-Ⅰ in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Pathways associated with ELISA Kit
| AMER1 Mutants Destabilize The Destruction Complex Pathway | AMER1 Mutants Destabilize The Destruction Complex Pathway |
| APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway | APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway |
| APC Truncation Mutants Have Impaired AXIN Binding Pathway | APC Truncation Mutants Have Impaired AXIN Binding Pathway |
| APC-Cdc20 Mediated Degradation Of Nek2A Pathway | APC-Cdc20 Mediated Degradation Of Nek2A Pathway |
| APC/C-mediated Degradation Of Cell Cycle Proteins Pathway | APC/C-mediated Degradation Of Cell Cycle Proteins Pathway |
| APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway | APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway |
| APC/C:Cdc20 Mediated Degradation Of Securin Pathway | APC/C:Cdc20 Mediated Degradation Of Securin Pathway |
| APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway | APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway |
| APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway | APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway |
| APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway | APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway |
Organs/Tissues associated with Ub ELISA Kit
| Gonad | publications with Ub and Gonad |
| Testis | publications with Ub and Testis |
