KEY FEATURES
| Full Name | Neurotrophin 3 |
|---|---|
| Synonym | NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 78-5000pg/mL |
| Sensitivity | 28pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse NT3 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NT3. You can calculate the concentration of Mouse NT3 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse NT3 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 248.82 | 764.13 | 1800.82 | 248.54 | 701.48 | 1964.34 |
| Standard deviation | 12.76 | 31.71 | 85.36 | 16.58 | 37.67 | 96.84 |
| CV(%) | 5.13 | 4.15 | 4.74 | 6.67 | 5.37 | 4.93 |
RECOVERY
The recovery of Mouse NT3 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-104 | 96 |
| EDTA plasma (n=8) | 87-101 | 93 |
| Cell culture media (n=8) | 91-104 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse NT3 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with NT3 ELISA Kits
| BDNF ELISA Kit | publications with NT3 and BDNF |
| NTRK3 ELISA Kit | publications with NT3 and NTRK3 |
| NTRK1 ELISA Kit | publications with NT3 and NTRK1 |
| NTRK2 ELISA Kit | publications with NT3 and NTRK2 |
| NGFR ELISA Kit | publications with NT3 and NGFR |
| HRAS ELISA Kit | publications with NT3 and HRAS |
| CREB1 ELISA Kit | publications with NT3 and CREB1 |
Pathways associated with NT3 ELISA Kit
| BDNF Signaling Pathway | BDNF Signaling Pathway |
| Integrated Pancreatic Cancer Pathway | Integrated Pancreatic Cancer Pathway |
| MAPK Signaling Pathway | MAPK Signaling Pathway |
| Neurotrophic Factor-mediated Trk Receptor Signaling Pathway | Neurotrophic Factor-mediated Trk Receptor Signaling Pathway |
| Neurotrophin Signaling Pathway | Neurotrophin Signaling Pathway |
| Trk Receptor Signaling Mediated By The MAPK Pathway | Trk Receptor Signaling Mediated By The MAPK Pathway |
| P75(NTR)-mediated Signaling Pathway | P75(NTR)-mediated Signaling Pathway |
Diseases associated with NT3 ELISA Kit
| Nervous System Diseases | publications with NT3 and Nervous System Diseases |
| Hearing Loss | publications with NT3 and Hearing Loss |
| Schizophrenia | publications with NT3 and Schizophrenia |
| Neoplasm Metastasis | publications with NT3 and Neoplasm Metastasis |
| Hypertension | publications with NT3 and Hypertension |
| Memory Disorders | publications with NT3 and Memory Disorders |
| Disease Models, Animal | publications with NT3 and Disease Models, Animal |
| Learning Disorders | publications with NT3 and Learning Disorders |
| Inflammation | publications with NT3 and Inflammation |
| Nerve Degeneration | publications with NT3 and Nerve Degeneration |
Organs/Tissues associated with NT3 ELISA Kit
| Brain | publications with NT3 and Brain |
| Testis | publications with NT3 and Testis |
| Embryonic Tissue | publications with NT3 and Embryonic Tissue |
| Eye | publications with NT3 and Eye |
| Ovary | publications with NT3 and Ovary |
