KEY FEATURES
| Full Name | Thyroid Stimulating Hormone |
|---|---|
| Synonym | Thyrotropin |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.3-120mIU/L |
| Sensitivity | 0.14mIU/L |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human TSH in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TSH. You can calculate the concentration of Human TSH in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human TSH ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (mIU/L) | 6.33 | 18.25 | 44.02 | 5.89 | 19.15 | 47.78 |
| Standard deviation | 0.34 | 1 | 1.95 | 0.36 | 1.11 | 1.96 |
| CV(%) | 5.42 | 5.5 | 4.44 | 6.11 | 5.79 | 4.11 |
RECOVERY
The recovery of Human TSH spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-109 | 99 |
| EDTA plasma (n=8) | 83-98 | 90 |
| Cell culture media (n=8) | 87-99 | 92 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human TSH in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CG ELISA Kits
| LHB ELISA Kit | publications with CG and LHB |
| FSHB ELISA Kit | publications with CG and FSHB |
| FSHR ELISA Kit | publications with CG and FSHR |
| TSHB ELISA Kit | publications with CG and TSHB |
| LHCGR ELISA Kit | publications with CG and LHCGR |
| CGB8 ELISA Kit | publications with CG and CGB8 |
Pathways associated with CG ELISA Kit
| Amine-derived Hormones Pathway | Amine-derived Hormones Pathway |
| Androgen Biosynthesis Pathway | Androgen Biosynthesis Pathway |
| Autoimmune Thyroid Disease Pathway | Autoimmune Thyroid Disease Pathway |
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| FSH Signaling Pathway | FSH Signaling Pathway |
| G Alpha (s) Signalling Events Pathway | G Alpha (s) Signalling Events Pathway |
| GPCR Downstream Signaling Pathway | GPCR Downstream Signaling Pathway |
| GPCR Ligand Binding Pathway | GPCR Ligand Binding Pathway |
| Glucocorticoid Receptor Regulatory Network Pathway | Glucocorticoid Receptor Regulatory Network Pathway |
Diseases associated with CG ELISA Kit
| Adenocarcinoma | publications with CG and Adenocarcinoma |
| Nervous System Diseases | publications with CG and Nervous System Diseases |
| Prostatic Diseases | publications with CG and Prostatic Diseases |
| Prostatic Neoplasms | publications with CG and Prostatic Neoplasms |
| Lung Neoplasms | publications with CG and Lung Neoplasms |
| Brain Diseases | publications with CG and Brain Diseases |
| Cardiovascular Diseases | publications with CG and Cardiovascular Diseases |
| Disease Progression | publications with CG and Disease Progression |
| Hyperplasia | publications with CG and Hyperplasia |
| Liver Neoplasms | publications with CG and Liver Neoplasms |
Organs/Tissues associated with CG ELISA Kit
| Brain | publications with CG and Brain |
| Lung | publications with CG and Lung |
| Stomach | publications with CG and Stomach |
| Liver | publications with CG and Liver |
| Thyroid | publications with CG and Thyroid |
| Heart | publications with CG and Heart |
| Muscle | publications with CG and Muscle |
| Vascular | publications with CG and Vascular |
| Pituitary Gland | publications with CG and Pituitary Gland |
| Skin | publications with CG and Skin |
