KEY FEATURES
| Full Name | Leptin |
|---|---|
| Synonym | OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein |
| Assay Type | Sandwich |
| Reactivity | Rat |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.13ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Rat LEP in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LEP. You can calculate the concentration of Rat LEP in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Rat LEP ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.08 | 2.18 | 7.19 | 1 | 2.33 | 6.87 |
| Standard deviation | 0.06 | 0.11 | 0.35 | 0.06 | 0.11 | 0.3 |
| CV(%) | 5.29 | 4.92 | 4.93 | 6.4 | 4.88 | 4.3 |
RECOVERY
The recovery of Rat LEP spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-100 | 95 |
| EDTA plasma (n=8) | 87-97 | 92 |
| Cell culture media (n=8) | 87-101 | 93 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Rat LEP in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with OB ELISA Kits
| LEPR ELISA Kit | publications with OB and LEPR |
| STAT3 ELISA Kit | publications with OB and STAT3 |
| INS ELISA Kit | publications with OB and INS |
| GHRL ELISA Kit | publications with OB and GHRL |
| CRP ELISA Kit | publications with OB and CRP |
| JAK2 ELISA Kit | publications with OB and JAK2 |
| PTPN1 ELISA Kit | publications with OB and PTPN1 |
| SOCS3 ELISA Kit | publications with OB and SOCS3 |
Pathways associated with OB ELISA Kit
| AMPK Signaling Pathway | AMPK Signaling Pathway |
| Adipocytokine Signaling Pathway | Adipocytokine Signaling Pathway |
| Adipogenesis Pathway | Adipogenesis Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Developmental Biology Pathway | Developmental Biology Pathway |
| HIF-1-alpha Transcription Factor Network Pathway | HIF-1-alpha Transcription Factor Network Pathway |
Diseases associated with OB ELISA Kit
| Obesity | publications with OB and Obesity |
| Pain | publications with OB and Pain |
| Insulin Resistance | publications with OB and Insulin Resistance |
| Neoplasms | publications with OB and Neoplasms |
| Hyperinsulinism | publications with OB and Hyperinsulinism |
| Weight Gain | publications with OB and Weight Gain |
| Weight Loss | publications with OB and Weight Loss |
| Cardiovascular Diseases | publications with OB and Cardiovascular Diseases |
| Fatty Liver | publications with OB and Fatty Liver |
| Inflammation | publications with OB and Inflammation |
Organs/Tissues associated with OB ELISA Kit
| Blood | publications with OB and Blood |
| Adipose Tissue | publications with OB and Adipose Tissue |
| Muscle | publications with OB and Muscle |
| Connective Tissue | publications with OB and Connective Tissue |
| Placenta | publications with OB and Placenta |
| Intestine | publications with OB and Intestine |
| Mouth | publications with OB and Mouth |
| Mammary Gland | publications with OB and Mammary Gland |
| Thymus | publications with OB and Thymus |
