Histone H2A.X Rabbit Monoclonal Antibody-EnkiLife

Histone H2A.X Rabbit Monoclonal Antibody

Summary

Production Name	Histone H2A.X Rabbit Monoclonal Antibody
Description	Recombinant rabbit monoclonal antibody
Host	Rabbit
Application	WB,IHC,ICC/IF,IP
Reactivity	Human

Performance

Conjugation	Unconjugated
Modification	Unmodified
Isotype	IgG
Clonality	Monoclonal
Form	Liquid
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Buffer	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification	Affinity Purification

Immunogen

Gene Name	H2AX
Alternative Names	H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
Gene ID	3014
SwissProt ID	P16104

Application

Dilution Ratio	WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,IP 1:20-1:50
Molecular Weight	Calculated MW: 15 kDa; Observed MW: 15 kDa

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Research Area

Epigenetics and Nuclear Signaling