KEY FEATURES
| Full Name | Caspase 12 |
|---|---|
| Synonym | CASP12P1; Cysteinyl Aspartate Specific Proteinases 12; Apoptosis-Related Cysteine Peptidase |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.014-10ng/mL |
| Sensitivity | 0.005ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CASP12 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CASP12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP12. You can calculate the concentration of Human CASP12 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CASP12 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.47 | 1.28 | 4.66 | 0.46 | 1.3 | 4.73 |
| Standard deviation | 0.02 | 0.07 | 0.17 | 0.03 | 0.07 | 0.22 |
| CV(%) | 5.29 | 5.58 | 3.69 | 6.07 | 5.28 | 4.64 |
RECOVERY
The recovery of Human CASP12 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-111 | 101 |
| EDTA plasma (n=8) | 96-112 | 102 |
| Cell culture media (n=8) | 89-104 | 95 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CASP12 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CASP12 ELISA Kits
| CASP3 ELISA Kit | publications with CASP12 and CASP3 |
| CAPN1 ELISA Kit | publications with CASP12 and CAPN1 |
| CAPN2 ELISA Kit | publications with CASP12 and CAPN2 |
Pathways associated with CASP12 ELISA Kit
| Alzheimer's Disease Pathway | Alzheimer's Disease Pathway |
| Amyotrophic Lateral Sclerosis (ALS) Pathway | Amyotrophic Lateral Sclerosis (ALS) Pathway |
| Apoptosis Pathway | Apoptosis Pathway |
| Hepatitis B Pathway | Hepatitis B Pathway |
| Prion Diseases Pathway | Prion Diseases Pathway |
| Protein Processing In Endoplasmic Reticulum Pathway | Protein Processing In Endoplasmic Reticulum Pathway |
Diseases associated with CASP12 ELISA Kit
| Nervous System Diseases | publications with CASP12 and Nervous System Diseases |
| Inflammation | publications with CASP12 and Inflammation |
| Brain Diseases | publications with CASP12 and Brain Diseases |
| Drug Toxicity | publications with CASP12 and Drug Toxicity |
| Neoplasms | publications with CASP12 and Neoplasms |
| Necrosis | publications with CASP12 and Necrosis |
| Kidney Diseases | publications with CASP12 and Kidney Diseases |
| Heart Diseases | publications with CASP12 and Heart Diseases |
| Liver Diseases | publications with CASP12 and Liver Diseases |
| Nerve Degeneration | publications with CASP12 and Nerve Degeneration |
Organs/Tissues associated with CASP12 ELISA Kit
| Kidney | publications with CASP12 and Kidney |
