KEY FEATURES
| Full Name | Receptor Activator Of Nuclear Factor Kappa B Ligand |
|---|---|
| Synonym | CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 15.6-1000pg/mL |
| Sensitivity | 5.8pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse RANkL in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RANkL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RANkL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RANkL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse RANkL. You can calculate the concentration of Mouse RANkL in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse RANkL ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 46.35 | 160.24 | 484.84 | 46.39 | 147.87 | 513.81 |
| Standard deviation | 2.98 | 7.48 | 22.11 | 3.17 | 6.74 | 17.57 |
| CV(%) | 6.43 | 4.67 | 4.56 | 6.83 | 4.56 | 3.42 |
RECOVERY
The recovery of Mouse RANkL spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-96 | 90 |
| EDTA plasma (n=8) | 84-97 | 91 |
| Cell culture media (n=8) | 91-107 | 99 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse RANkL in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Pathways associated with ELISA Kit
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| NF-kappa B Signaling Pathway | NF-kappa B Signaling Pathway |
| Osteoblast Pathway | Osteoblast Pathway |
| Osteoclast Pathway | Osteoclast Pathway |
| Osteoclast Differentiation Pathway | Osteoclast Differentiation Pathway |
| Prolactin Signaling Pathway | Prolactin Signaling Pathway |
| Rheumatoid Arthritis Pathway | Rheumatoid Arthritis Pathway |
Diseases associated with ODF ELISA Kit
| Necrosis | publications with ODF and Necrosis |
| Osteoporosis | publications with ODF and Osteoporosis |
| Neoplasms | publications with ODF and Neoplasms |
| Inflammation | publications with ODF and Inflammation |
| Osteolysis | publications with ODF and Osteolysis |
| Cardiovascular Diseases | publications with ODF and Cardiovascular Diseases |
| Breast Neoplasms | publications with ODF and Breast Neoplasms |
| Alveolar Bone Loss | publications with ODF and Alveolar Bone Loss |
| Osteopetrosis | publications with ODF and Osteopetrosis |
| Pain | publications with ODF and Pain |
Organs/Tissues associated with ODF ELISA Kit
| Bone | publications with ODF and Bone |
| Spleen | publications with ODF and Spleen |
| Thymus | publications with ODF and Thymus |
