KEY FEATURES
| Full Name | Platelet Factor 4 |
|---|---|
| Synonym | CXCL4 |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 0.16-10ng/mL |
| Sensitivity | 0.07ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse PF4 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PF4. You can calculate the concentration of Mouse PF4 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse PF4 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.49 | 1.31 | 3.87 | 0.45 | 1.37 | 3.81 |
| Standard deviation | 0.03 | 0.06 | 0.12 | 0.03 | 0.06 | 0.18 |
| CV(%) | 5.44 | 4.42 | 3.05 | 6.33 | 4.09 | 4.85 |
RECOVERY
The recovery of Mouse PF4 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-101 | 93 |
| EDTA plasma (n=8) | 94-106 | 101 |
| Cell culture media (n=8) | 89-99 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse PF4 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with PF4 ELISA Kits
| FGF2 ELISA Kit | publications with PF4 and FGF2 |
| CXCR3 ELISA Kit | publications with PF4 and CXCR3 |
| PROC ELISA Kit | publications with PF4 and PROC |
| CXCL11 ELISA Kit | publications with PF4 and CXCL11 |
Pathways associated with PF4 ELISA Kit
| CXCR3-mediated Signaling Events Pathway | CXCR3-mediated Signaling Events Pathway |
| Cell Surface Interactions At The Vascular Wall Pathway | Cell Surface Interactions At The Vascular Wall Pathway |
| Chemokine Receptors Bind Chemokines Pathway | Chemokine Receptors Bind Chemokines Pathway |
| Chemokine Signaling Pathway | Chemokine Signaling Pathway |
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| Common Pathway | Common Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Formation Of Fibrin Clot (Clotting Cascade) Pathway | Formation Of Fibrin Clot (Clotting Cascade) Pathway |
Diseases associated with PF4 ELISA Kit
| Thrombocytopenia | publications with PF4 and Thrombocytopenia |
| Cardiovascular Diseases | publications with PF4 and Cardiovascular Diseases |
| Thrombosis | publications with PF4 and Thrombosis |
| Heart Diseases | publications with PF4 and Heart Diseases |
| Neoplasms | publications with PF4 and Neoplasms |
| Inflammation | publications with PF4 and Inflammation |
| Nervous System Diseases | publications with PF4 and Nervous System Diseases |
| Autoimmune Diseases | publications with PF4 and Autoimmune Diseases |
| Kidney Diseases | publications with PF4 and Kidney Diseases |
| Cerebral Infarction | publications with PF4 and Cerebral Infarction |
Organs/Tissues associated with PF4 ELISA Kit
| Heart | publications with PF4 and Heart |
| Bone | publications with PF4 and Bone |
| Bone Marrow | publications with PF4 and Bone Marrow |
| Skin | publications with PF4 and Skin |
| Lung | publications with PF4 and Lung |
| Embryonic Tissue | publications with PF4 and Embryonic Tissue |
| Intestine | publications with PF4 and Intestine |
| Placenta | publications with PF4 and Placenta |
