KEY FEATURES
| Full Name | Gastric Inhibitory Polypeptide |
|---|---|
| Synonym | Incretin hormone; Glucose Dependent Insulinotropic Peptide |
| Assay Type | Competitive |
| Reactivity | Mouse |
| Range | 61.7-5000pg/mL |
| Sensitivity | 20.9pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 50μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse GIP in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse GIP. During the reaction, Mouse GIP in the sample or standard competes with a fixed amount of Mouse GIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse GIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse GIP in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse GIP ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 261.1 | 582.39 | 1762.75 | 274.53 | 562.02 | 1709.9 |
| Standard deviation | 14.36 | 34.54 | 52.88 | 19 | 33.33 | 80.19 |
| CV(%) | 5.5 | 5.93 | 3 | 6.92 | 5.93 | 4.69 |
RECOVERY
The recovery of Mouse GIP spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-104 | 95 |
| EDTA plasma (n=8) | 93-104 | 98 |
| Cell culture media (n=8) | 87-99 | 92 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse GIP in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with GIP ELISA Kits
| GIPR ELISA Kit | publications with GIP and GIPR |
| DPP4 ELISA Kit | publications with GIP and DPP4 |
| BAX ELISA Kit | publications with GIP and BAX |
| GATA4 ELISA Kit | publications with GIP and GATA4 |
| RETN ELISA Kit | publications with GIP and RETN |
| ISL1 ELISA Kit | publications with GIP and ISL1 |
Pathways associated with GIP ELISA Kit
| Class B/2 (Secretin Family Receptors) Pathway | Class B/2 (Secretin Family Receptors) Pathway |
| Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway | Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway |
| Disease Pathway | Disease Pathway |
| G Alpha (s) Signalling Events Pathway | G Alpha (s) Signalling Events Pathway |
| GPCR Downstream Signaling Pathway | GPCR Downstream Signaling Pathway |
| GPCR Ligand Binding Pathway | GPCR Ligand Binding Pathway |
| Glucagon-type Ligand Receptors Pathway | Glucagon-type Ligand Receptors Pathway |
| Incretin Synthesis, Secretion, And Inactivation Pathway | Incretin Synthesis, Secretion, And Inactivation Pathway |
| Insulin Secretion Pathway | Insulin Secretion Pathway |
| Metabolic Disorders Of Biological Oxidation Enzymes Pathway | Metabolic Disorders Of Biological Oxidation Enzymes Pathway |
Diseases associated with GIP ELISA Kit
| Diabetes Mellitus, Type 2 | publications with GIP and Diabetes Mellitus, Type 2 |
| Weight Loss | publications with GIP and Weight Loss |
| Cardiovascular Diseases | publications with GIP and Cardiovascular Diseases |
| Weight Gain | publications with GIP and Weight Gain |
| Carcinoma | publications with GIP and Carcinoma |
| Hyperplasia | publications with GIP and Hyperplasia |
| Kidney Diseases | publications with GIP and Kidney Diseases |
| Hypertension | publications with GIP and Hypertension |
| Necrosis | publications with GIP and Necrosis |
| Fatty Liver | publications with GIP and Fatty Liver |
Organs/Tissues associated with GIP ELISA Kit
| Intestine | publications with GIP and Intestine |
| Stomach | publications with GIP and Stomach |
