KEY FEATURES
| Full Name | Glutamate Receptor Interacting Protein 1 |
|---|---|
| Synonym | GRIP1 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.13ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human GRIP1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GRIP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GRIP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRIP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GRIP1. You can calculate the concentration of Human GRIP1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human GRIP1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1 | 2.27 | 8.37 | 1.09 | 2.41 | 8.2 |
| Standard deviation | 0.06 | 0.13 | 0.34 | 0.07 | 0.11 | 0.41 |
| CV(%) | 6.43 | 5.86 | 4.03 | 6.24 | 4.41 | 4.99 |
RECOVERY
The recovery of Human GRIP1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-103 | 95 |
| EDTA plasma (n=8) | 90-106 | 98 |
| Cell culture media (n=8) | 86-97 | 92 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human GRIP1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with GRIP1 ELISA Kits
| PICK1 ELISA Kit | publications with GRIP1 and PICK1 |
| CARM1 ELISA Kit | publications with GRIP1 and CARM1 |
| EP300 ELISA Kit | publications with GRIP1 and EP300 |
| GRIK2 ELISA Kit | publications with GRIP1 and GRIK2 |
| GRIK1 ELISA Kit | publications with GRIP1 and GRIK1 |
| NR3C1 ELISA Kit | publications with GRIP1 and NR3C1 |
Pathways associated with GRIP1 ELISA Kit
| BDNF Signaling Pathway | BDNF Signaling Pathway |
| Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway | Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway |
| Neuronal System Pathway | Neuronal System Pathway |
| Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway | Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway |
| Trafficking Of AMPA Receptors Pathway | Trafficking Of AMPA Receptors Pathway |
| Trafficking Of GluR2-containing AMPA Receptors Pathway | Trafficking Of GluR2-containing AMPA Receptors Pathway |
| Transmission Across Chemical Synapses Pathway | Transmission Across Chemical Synapses Pathway |
Diseases associated with GRIP1 ELISA Kit
| Genital Diseases, Male | publications with GRIP1 and Genital Diseases, Male |
| Necrosis | publications with GRIP1 and Necrosis |
| Congenital Abnormalities | publications with GRIP1 and Congenital Abnormalities |
| Inflammation | publications with GRIP1 and Inflammation |
| Disease Models, Animal | publications with GRIP1 and Disease Models, Animal |
| Eye Abnormalities | publications with GRIP1 and Eye Abnormalities |
| Learning Disorders | publications with GRIP1 and Learning Disorders |
| Memory Disorders | publications with GRIP1 and Memory Disorders |
| Liver Diseases | publications with GRIP1 and Liver Diseases |
| Cardiovascular Diseases | publications with GRIP1 and Cardiovascular Diseases |
Organs/Tissues associated with GRIP1 ELISA Kit
| Brain | publications with GRIP1 and Brain |
| Embryonic Tissue | publications with GRIP1 and Embryonic Tissue |
| Kidney | publications with GRIP1 and Kidney |
| Testis | publications with GRIP1 and Testis |
| Eye | publications with GRIP1 and Eye |
| Heart | publications with GRIP1 and Heart |
| Skin | publications with GRIP1 and Skin |
| Ovary | publications with GRIP1 and Ovary |
| Uncharacterized Tissue | publications with GRIP1 and Uncharacterized Tissue |
| Placenta | publications with GRIP1 and Placenta |
