KEY FEATURES
| Full Name | Cluster Of Differentiation 74 |
|---|---|
| Synonym | MHCDG; HLA-DG; HLADG; DHLAG; Ia-GAMMA; HLA class II histocompatibility antigen gamma chain; Ia antigen-associated invariant chain; HLA-DR antigens-associated invariant chain |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 46.88-3000pg/mL |
| Sensitivity | 17.61pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CD74 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD74. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD74 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD74, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD74. You can calculate the concentration of Human CD74 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CD74 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 150.06 | 421.78 | 1128.19 | 142.76 | 388.75 | 1030.8 |
| Standard deviation | 7.64 | 17.88 | 48.06 | 7.82 | 17.65 | 42.57 |
| CV(%) | 5.09 | 4.24 | 4.26 | 5.48 | 4.54 | 4.13 |
RECOVERY
The recovery of Human CD74 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-104 | 99 |
| EDTA plasma (n=8) | 90-104 | 97 |
| Cell culture media (n=8) | 86-102 | 93 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CD74 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CD74 ELISA Kits
| MIF ELISA Kit | publications with CD74 and MIF |
| HLA-DRB1 ELISA Kit | publications with CD74 and HLA-DRB1 |
Pathways associated with CD74 ELISA Kit
| Adaptive Immune System Pathway | Adaptive Immune System Pathway |
| Antigen Processing And Presentation Pathway | Antigen Processing And Presentation Pathway |
| Cell Surface Interactions At The Vascular Wall Pathway | Cell Surface Interactions At The Vascular Wall Pathway |
| Hemostasis Pathway | Hemostasis Pathway |
| Herpes Simplex Infection Pathway | Herpes Simplex Infection Pathway |
| Immune System Pathway | Immune System Pathway |
| MHC Class II Antigen Presentation Pathway | MHC Class II Antigen Presentation Pathway |
| Tuberculosis Pathway | Tuberculosis Pathway |
Diseases associated with CD74 ELISA Kit
| Immune System Diseases | publications with CD74 and Immune System Diseases |
| Inflammation | publications with CD74 and Inflammation |
| Carcinoma | publications with CD74 and Carcinoma |
| Disease Models, Animal | publications with CD74 and Disease Models, Animal |
| Cardiovascular Diseases | publications with CD74 and Cardiovascular Diseases |
| Lung Neoplasms | publications with CD74 and Lung Neoplasms |
| Brain Diseases | publications with CD74 and Brain Diseases |
| Necrosis | publications with CD74 and Necrosis |
| Carcinoma, Non-Small-Cell Lung | publications with CD74 and Carcinoma, Non-Small-Cell Lung |
| Kidney Diseases | publications with CD74 and Kidney Diseases |
Organs/Tissues associated with CD74 ELISA Kit
| Blood | publications with CD74 and Blood |
| Lung | publications with CD74 and Lung |
| Brain | publications with CD74 and Brain |
| Bone | publications with CD74 and Bone |
| Bone Marrow | publications with CD74 and Bone Marrow |
| Vascular | publications with CD74 and Vascular |
| Lymph | publications with CD74 and Lymph |
| Lymph Node | publications with CD74 and Lymph Node |
| Kidney | publications with CD74 and Kidney |
| Skin | publications with CD74 and Skin |
