KEY FEATURES
| Full Name | Cytokeratin 14 |
|---|---|
| Synonym | KRT14; EBS3; EBS4; K14; Keratin 14; Epidermolysis Bullosa Simplex Dowling-Meara Koebner; Keratin, type I cytoskeletal 14 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CK14 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CK14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CK14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CK14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CK14. You can calculate the concentration of Human CK14 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CK14 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.49 | 1.2 | 4.97 | 0.47 | 1.18 | 4.49 |
| Standard deviation | 0.03 | 0.07 | 0.27 | 0.03 | 0.05 | 0.19 |
| CV(%) | 5.7 | 5.79 | 5.45 | 6.65 | 4.31 | 4.29 |
RECOVERY
The recovery of Human CK14 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-102 | 96 |
| EDTA plasma (n=8) | 94-107 | 100 |
| Cell culture media (n=8) | 90-104 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CK14 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with EK ELISA Kits
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| EGFR ELISA Kit | publications with EK and EGFR |
| DST ELISA Kit | publications with EK and DST |
| IVL ELISA Kit | publications with EK and IVL |
| CREBBP ELISA Kit | publications with EK and CREBBP |
Pathways associated with EK ELISA Kit
| Corticotropin-releasing Hormone Pathway | Corticotropin-releasing Hormone Pathway |
| Glucocorticoid Receptor Regulatory Network Pathway | Glucocorticoid Receptor Regulatory Network Pathway |
Diseases associated with EK ELISA Kit
| Epidermolysis Bullosa Simplex | publications with EK and Epidermolysis Bullosa Simplex |
| Carcinoma | publications with EK and Carcinoma |
| Carcinoma, Squamous Cell | publications with EK and Carcinoma, Squamous Cell |
| Disease Models, Animal | publications with EK and Disease Models, Animal |
| Hyperplasia | publications with EK and Hyperplasia |
| Breast Neoplasms | publications with EK and Breast Neoplasms |
| Necrosis | publications with EK and Necrosis |
| Cell Transformation, Neoplastic | publications with EK and Cell Transformation, Neoplastic |
| Inflammation | publications with EK and Inflammation |
| Neoplasms, Experimental | publications with EK and Neoplasms, Experimental |
Organs/Tissues associated with EK ELISA Kit
| Skin | publications with EK and Skin |
| Mouth | publications with EK and Mouth |
| Lung | publications with EK and Lung |
| Esophagus | publications with EK and Esophagus |
| Eye | publications with EK and Eye |
| Mammary Gland | publications with EK and Mammary Gland |
| Connective Tissue | publications with EK and Connective Tissue |
| Liver | publications with EK and Liver |
| Cervix | publications with EK and Cervix |
| Bladder | publications with EK and Bladder |
