KEY FEATURES
| Full Name | Cytokeratin 18 |
|---|---|
| Synonym | KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CK18 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CK18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CK18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CK18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CK18. You can calculate the concentration of Human CK18 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CK18 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.52 | 0.97 | 3.69 | 0.48 | 0.96 | 3.76 |
| Standard deviation | 0.03 | 0.04 | 0.18 | 0.03 | 0.05 | 0.2 |
| CV(%) | 6.25 | 4.31 | 4.89 | 5.4 | 4.82 | 5.44 |
RECOVERY
The recovery of Human CK18 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-103 | 94 |
| EDTA plasma (n=8) | 87-99 | 92 |
| Cell culture media (n=8) | 90-104 | 95 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CK18 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with Krt18 ELISA Kits
| Krt8 ELISA Kit | publications with Krt18 and Krt8 |
| Krt14 ELISA Kit | publications with Krt18 and Krt14 |
| Krt5 ELISA Kit | publications with Krt18 and Krt5 |
| Krt4 ELISA Kit | publications with Krt18 and Krt4 |
| Tchp ELISA Kit | publications with Krt18 and Tchp |
| Ywhaz ELISA Kit | publications with Krt18 and Ywhaz |
| Krt1 ELISA Kit | publications with Krt18 and Krt1 |
Pathways associated with Krt18 ELISA Kit
| Developmental Biology Pathway | Developmental Biology Pathway |
| EGFR1 Signaling Pathway | EGFR1 Signaling Pathway |
| Keratinization Pathway | Keratinization Pathway |
Diseases associated with Krt18 ELISA Kit
| Liver Diseases | publications with Krt18 and Liver Diseases |
| Fibrosis | publications with Krt18 and Fibrosis |
| Fatty Liver | publications with Krt18 and Fatty Liver |
| Adenocarcinoma | publications with Krt18 and Adenocarcinoma |
| Breast Neoplasms | publications with Krt18 and Breast Neoplasms |
| Neoplasm Metastasis | publications with Krt18 and Neoplasm Metastasis |
| Inflammation | publications with Krt18 and Inflammation |
| Liver Cirrhosis | publications with Krt18 and Liver Cirrhosis |
| Disease Models, Animal | publications with Krt18 and Disease Models, Animal |
| Necrosis | publications with Krt18 and Necrosis |
Organs/Tissues associated with Krt18 ELISA Kit
| Liver | publications with Krt18 and Liver |
| Prostate | publications with Krt18 and Prostate |
| Bone | publications with Krt18 and Bone |
| Kidney | publications with Krt18 and Kidney |
| Eye | publications with Krt18 and Eye |
| Stomach | publications with Krt18 and Stomach |
| Embryonic Tissue | publications with Krt18 and Embryonic Tissue |
| Muscle | publications with Krt18 and Muscle |
| Intestine | publications with Krt18 and Intestine |
| Brain | publications with Krt18 and Brain |
