KEY FEATURES
| Full Name | Probable ATP-dependent RNA Helicase DDX58 |
|---|---|
| Synonym | RIG-I; RIGI; DEAD(Asp-Glu-Ala-Asp)box Polypeptide 58; Probable ATP-Dependent RNA Helicase DDX58; RIG-I-like receptor; RIG-I-like receptor 1 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.12ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human DDX58 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DDX58. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DDX58 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DDX58, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DDX58. You can calculate the concentration of Human DDX58 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human DDX58 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.91 | 2.27 | 7.63 | 0.9 | 2.19 | 6.95 |
| Standard deviation | 0.06 | 0.13 | 0.24 | 0.05 | 0.09 | 0.3 |
| CV(%) | 6.5 | 5.85 | 3.19 | 5.08 | 4.23 | 4.38 |
RECOVERY
The recovery of Human DDX58 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-104 | 97 |
| EDTA plasma (n=8) | 88-103 | 96 |
| Cell culture media (n=8) | 91-105 | 98 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human DDX58 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with DDX58 ELISA Kits
| IFIH1 ELISA Kit | publications with DDX58 and IFIH1 |
| MAVS ELISA Kit | publications with DDX58 and MAVS |
| TRIM25 ELISA Kit | publications with DDX58 and TRIM25 |
| ISG15 ELISA Kit | publications with DDX58 and ISG15 |
| IFIT1 ELISA Kit | publications with DDX58 and IFIT1 |
| CYLD ELISA Kit | publications with DDX58 and CYLD |
| RNF135 ELISA Kit | publications with DDX58 and RNF135 |
| UBE2L6 ELISA Kit | publications with DDX58 and UBE2L6 |
Pathways associated with DDX58 ELISA Kit
| Antiviral Mechanism By IFN-stimulated Genes Pathway | Antiviral Mechanism By IFN-stimulated Genes Pathway |
| Cytokine Signaling In Immune System Pathway | Cytokine Signaling In Immune System Pathway |
| Cytosolic DNA-sensing Pathway | Cytosolic DNA-sensing Pathway |
| Epstein-Barr Virus Infection Pathway | Epstein-Barr Virus Infection Pathway |
| Hepatitis B Pathway | Hepatitis B Pathway |
| Hepatitis C Pathway | Hepatitis C Pathway |
| Herpes Simplex Infection Pathway | Herpes Simplex Infection Pathway |
Diseases associated with DDX58 ELISA Kit
| Inflammation | publications with DDX58 and Inflammation |
| Hepatitis C | publications with DDX58 and Hepatitis C |
| Influenza, Human | publications with DDX58 and Influenza, Human |
| Necrosis | publications with DDX58 and Necrosis |
| Liver Diseases | publications with DDX58 and Liver Diseases |
| Nervous System Diseases | publications with DDX58 and Nervous System Diseases |
| Lung Diseases | publications with DDX58 and Lung Diseases |
| Disease Models, Animal | publications with DDX58 and Disease Models, Animal |
| Kidney Diseases | publications with DDX58 and Kidney Diseases |
| Carcinoma, Hepatocellular | publications with DDX58 and Carcinoma, Hepatocellular |
Organs/Tissues associated with DDX58 ELISA Kit
| Blood | publications with DDX58 and Blood |
| Lung | publications with DDX58 and Lung |
| Vascular | publications with DDX58 and Vascular |
| Kidney | publications with DDX58 and Kidney |
| Liver | publications with DDX58 and Liver |
| Brain | publications with DDX58 and Brain |
| Bone | publications with DDX58 and Bone |
| Nerve | publications with DDX58 and Nerve |
| Bone Marrow | publications with DDX58 and Bone Marrow |
| Skin | publications with DDX58 and Skin |
