KEY FEATURES
| Full Name | Relaxin |
|---|---|
| Synonym | RLXH1; Relaxin H1; Prorelaxin H1 |
| Assay Type | Competitive |
| Reactivity | Human |
| Range | 3.2-2000pg/mL |
| Sensitivity | 1.4pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 50μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human RLN in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human RLN. During the reaction, Human RLN in the sample or standard competes with a fixed amount of Human RLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human RLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human RLN in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human RLN ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 93.76 | 195.51 | 936.06 | 89.86 | 187.89 | 868.41 |
| Standard deviation | 5.13 | 9.21 | 40.44 | 5.08 | 7.84 | 39.6 |
| CV(%) | 5.47 | 4.71 | 4.32 | 5.65 | 4.17 | 4.56 |
RECOVERY
The recovery of Human RLN spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-100 | 93 |
| EDTA plasma (n=8) | 93-103 | 98 |
| Cell culture media (n=8) | 91-105 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human RLN in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with RLN ELISA Kits
| RLN3 ELISA Kit | publications with RLN and RLN3 |
| RXFP1 ELISA Kit | publications with RLN and RXFP1 |
| INSL3 ELISA Kit | publications with RLN and INSL3 |
| RXFP4 ELISA Kit | publications with RLN and RXFP4 |
| RXFP2 ELISA Kit | publications with RLN and RXFP2 |
| INSL5 ELISA Kit | publications with RLN and INSL5 |
Pathways associated with RLN ELISA Kit
| Androgen Receptor Signaling Pathway | Androgen Receptor Signaling Pathway |
| Myometrial Relaxation And Contraction Pathways | Myometrial Relaxation And Contraction Pathways |
Diseases associated with RLN ELISA Kit
| Fibrosis | publications with RLN and Fibrosis |
| Heart Diseases | publications with RLN and Heart Diseases |
| Death | publications with RLN and Death |
| Genital Diseases, Male | publications with RLN and Genital Diseases, Male |
| Lung Diseases | publications with RLN and Lung Diseases |
| Disease Models, Animal | publications with RLN and Disease Models, Animal |
| Hypertrophy | publications with RLN and Hypertrophy |
| Prostatic Diseases | publications with RLN and Prostatic Diseases |
| Nervous System Diseases | publications with RLN and Nervous System Diseases |
| Prostatic Neoplasms | publications with RLN and Prostatic Neoplasms |
Organs/Tissues associated with RLN ELISA Kit
| Brain | publications with RLN and Brain |
| Blood | publications with RLN and Blood |
| Prostate | publications with RLN and Prostate |
| Connective Tissue | publications with RLN and Connective Tissue |
| Testis | publications with RLN and Testis |
