KEY FEATURES
| Full Name | Neurofilament, Light Polypeptide |
|---|---|
| Synonym | NEFL |
| Assay Type | Sandwich |
| Reactivity | Monkey |
| Range | 31.25-2000pg/mL |
| Sensitivity | 18.75pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Monkey NEFL in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey NEFL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey NEFL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey NEFL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey NEFL. You can calculate the concentration of Monkey NEFL in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Monkey NEFL ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 95.42 | 225.28 | 739.8 | 94.14 | 226.21 | 764.05 |
| Standard deviation | 5.54 | 11.85 | 34.55 | 5.54 | 9.16 | 35.3 |
| CV(%) | 5.81 | 5.26 | 4.67 | 5.88 | 4.05 | 4.62 |
RECOVERY
The recovery of Monkey NEFL spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-104 | 99 |
| EDTA plasma (n=8) | 90-103 | 97 |
| Cell culture media (n=8) | 89-102 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Monkey NEFL in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with NEFL ELISA Kits
| MTMR2 ELISA Kit | publications with NEFL and MTMR2 |
| GAN ELISA Kit | publications with NEFL and GAN |
| NEFH ELISA Kit | publications with NEFL and NEFH |
| NEFM ELISA Kit | publications with NEFL and NEFM |
| TSC1 ELISA Kit | publications with NEFL and TSC1 |
Pathways associated with NEFL ELISA Kit
| Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway | Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway |
| Amyotrophic Lateral Sclerosis (ALS) Pathway | Amyotrophic Lateral Sclerosis (ALS) Pathway |
| CREB Phosphorylation Through The Activation Of CaMKII Pathway | CREB Phosphorylation Through The Activation Of CaMKII Pathway |
| CREB Phosphorylation Through The Activation Of Ras Pathway | CREB Phosphorylation Through The Activation Of Ras Pathway |
| Neuronal System Pathway | Neuronal System Pathway |
| Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway | Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway |
| Post NMDA Receptor Activation Events Pathway | Post NMDA Receptor Activation Events Pathway |
| Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway | Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway |
Diseases associated with NEFL ELISA Kit
| Nervous System Diseases | publications with NEFL and Nervous System Diseases |
| Nervous System Malformations | publications with NEFL and Nervous System Malformations |
| Breast Neoplasms | publications with NEFL and Breast Neoplasms |
| Brain Diseases | publications with NEFL and Brain Diseases |
| Disease Models, Animal | publications with NEFL and Disease Models, Animal |
| Charcot-Marie-Tooth disease, Type 1F | publications with NEFL and Charcot-Marie-Tooth disease, Type 1F |
| Hypertension | publications with NEFL and Hypertension |
| Lung Neoplasms | publications with NEFL and Lung Neoplasms |
| Ataxia | publications with NEFL and Ataxia |
| Immune System Diseases | publications with NEFL and Immune System Diseases |
Organs/Tissues associated with NEFL ELISA Kit
| Nerve | publications with NEFL and Nerve |
| Brain | publications with NEFL and Brain |
| Prostate | publications with NEFL and Prostate |
| Spinal Cord | publications with NEFL and Spinal Cord |
| Lung | publications with NEFL and Lung |
| Eye | publications with NEFL and Eye |
| Ganglia | publications with NEFL and Ganglia |
| Kidney | publications with NEFL and Kidney |
| Bone Marrow | publications with NEFL and Bone Marrow |
| Embryonic Tissue | publications with NEFL and Embryonic Tissue |
