KEY FEATURES
| Full Name | Laminin |
|---|---|
| Synonym | LAM |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 78-5000pg/mL |
| Sensitivity | 32pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse LN in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LN. You can calculate the concentration of Mouse LN in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse LN ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 266.21 | 657.33 | 1962.21 | 271.38 | 630.42 | 1876.91 |
| Standard deviation | 14.22 | 35.89 | 101.25 | 14.06 | 25.41 | 84.27 |
| CV(%) | 5.34 | 5.46 | 5.16 | 5.18 | 4.03 | 4.49 |
RECOVERY
The recovery of Mouse LN spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-105 | 98 |
| EDTA plasma (n=8) | 94-110 | 100 |
| Cell culture media (n=8) | 93-106 | 99 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse LN in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with LN ELISA Kits
| LAMB3 ELISA Kit | publications with LN and LAMB3 |
| LAMA3 ELISA Kit | publications with LN and LAMA3 |
| ITGB4 ELISA Kit | publications with LN and ITGB4 |
| COL17A1 ELISA Kit | publications with LN and COL17A1 |
| ITGB1 ELISA Kit | publications with LN and ITGB1 |
Pathways associated with LN ELISA Kit
| Alpha6-Beta4 Integrin Signaling Pathway | Alpha6-Beta4 Integrin Signaling Pathway |
| Amoebiasis Pathway | Amoebiasis Pathway |
| Anchoring Fibril Formation Pathway | Anchoring Fibril Formation Pathway |
| Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway | Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway |
| Cell Junction Organization Pathway | Cell Junction Organization Pathway |
| Cell-Cell Communication Pathway | Cell-Cell Communication Pathway |
| Collagen Formation Pathway | Collagen Formation Pathway |
| ECM-receptor Interaction Pathway | ECM-receptor Interaction Pathway |
Diseases associated with LN ELISA Kit
| Neoplasms | publications with LN and Neoplasms |
| Carcinoma | publications with LN and Carcinoma |
| Congenital Abnormalities | publications with LN and Congenital Abnormalities |
| Epidermolysis Bullosa, Junctional | publications with LN and Epidermolysis Bullosa, Junctional |
| Neoplasms, Experimental | publications with LN and Neoplasms, Experimental |
| Liver Neoplasms | publications with LN and Liver Neoplasms |
| Disease Models, Animal | publications with LN and Disease Models, Animal |
| Precancerous Conditions | publications with LN and Precancerous Conditions |
| Liver Diseases | publications with LN and Liver Diseases |
| Uterine Cervical Neoplasms | publications with LN and Uterine Cervical Neoplasms |
Organs/Tissues associated with LN ELISA Kit
| Skin | publications with LN and Skin |
| Mouth | publications with LN and Mouth |
| Lung | publications with LN and Lung |
| Lymph Node | publications with LN and Lymph Node |
| Embryonic Tissue | publications with LN and Embryonic Tissue |
| Liver | publications with LN and Liver |
| Stomach | publications with LN and Stomach |
| Bone | publications with LN and Bone |
| Nerve | publications with LN and Nerve |
| Bladder | publications with LN and Bladder |
