KEY FEATURES
| Full Name | Nephrin |
|---|---|
| Synonym | NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.13ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse NPHN in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NPHN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NPHN. You can calculate the concentration of Mouse NPHN in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse NPHN ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.96 | 2.64 | 7.51 | 1.02 | 2.85 | 7.26 |
| Standard deviation | 0.05 | 0.12 | 0.27 | 0.06 | 0.15 | 0.38 |
| CV(%) | 5.09 | 4.61 | 3.54 | 5.57 | 5.22 | 5.19 |
RECOVERY
The recovery of Mouse NPHN spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-109 | 100 |
| EDTA plasma (n=8) | 94-109 | 100 |
| Cell culture media (n=8) | 90-102 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse NPHN in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with NPHN ELISA Kits
| NPHS2 ELISA Kit | publications with NPHN and NPHS2 |
| CD2AP ELISA Kit | publications with NPHN and CD2AP |
| TRPC6 ELISA Kit | publications with NPHN and TRPC6 |
| TJP1 ELISA Kit | publications with NPHN and TJP1 |
| SRC ELISA Kit | publications with NPHN and SRC |
| KIRREL ELISA Kit | publications with NPHN and KIRREL |
| FYN ELISA Kit | publications with NPHN and FYN |
Pathways associated with NPHN ELISA Kit
| Cell-Cell Communication Pathway | Cell-Cell Communication Pathway |
| Nephrin Interactions Pathway | Nephrin Interactions Pathway |
| Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway | Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway |
Diseases associated with NPHN ELISA Kit
| Nephrotic Syndrome | publications with NPHN and Nephrotic Syndrome |
| Kidney Diseases | publications with NPHN and Kidney Diseases |
| Nephrosis | publications with NPHN and Nephrosis |
| Proteinuria | publications with NPHN and Proteinuria |
| Glomerulonephritis | publications with NPHN and Glomerulonephritis |
| Glomerulosclerosis, Focal Segmental | publications with NPHN and Glomerulosclerosis, Focal Segmental |
| Renal Insufficiency | publications with NPHN and Renal Insufficiency |
| Kidney Failure, Chronic | publications with NPHN and Kidney Failure, Chronic |
| Disease Models, Animal | publications with NPHN and Disease Models, Animal |
| Hypertension | publications with NPHN and Hypertension |
Organs/Tissues associated with NPHN ELISA Kit
| Kidney | publications with NPHN and Kidney |
| Pancreas | publications with NPHN and Pancreas |
| Brain | publications with NPHN and Brain |
| Thymus | publications with NPHN and Thymus |
| Testis | publications with NPHN and Testis |
