KEY FEATURES
| Full Name | S100 Calcium Binding Protein B |
|---|---|
| Synonym | S100-B;NEF;S100Beta; |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 78.13-5000pg/mL |
| Sensitivity | 46.88pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse S100B in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse S100B. You can calculate the concentration of Mouse S100B in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse S100B ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 259.59 | 527.1 | 2197.45 | 247.06 | 571.78 | 2018.49 |
| Standard deviation | 13.29 | 26.99 | 97.57 | 17.02 | 29.28 | 71.05 |
| CV(%) | 5.12 | 5.12 | 4.44 | 6.89 | 5.12 | 3.52 |
RECOVERY
The recovery of Mouse S100B spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-98 | 90 |
| EDTA plasma (n=8) | 89-104 | 95 |
| Cell culture media (n=8) | 84-98 | 90 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse S100B in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with S100 ELISA Kits
| GFAP ELISA Kit | publications with S100 and GFAP |
| S100A1 ELISA Kit | publications with S100 and S100A1 |
| APP ELISA Kit | publications with S100 and APP |
| TP53 ELISA Kit | publications with S100 and TP53 |
| IQGAP1 ELISA Kit | publications with S100 and IQGAP1 |
| AHNAK ELISA Kit | publications with S100 and AHNAK |
| ANXA6 ELISA Kit | publications with S100 and ANXA6 |
| STK38 ELISA Kit | publications with S100 and STK38 |
Pathways associated with S100 ELISA Kit
| Activated TLR4 Signalling Pathway | Activated TLR4 Signalling Pathway |
| Advanced Glycosylation Endproduct Receptor Signaling Pathway | Advanced Glycosylation Endproduct Receptor Signaling Pathway |
| Cytosolic Sensors Of Pathogen-associated DNA Pathway | Cytosolic Sensors Of Pathogen-associated DNA Pathway |
| DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway | DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway |
| Immune System Pathway | Immune System Pathway |
| Innate Immune System Pathway | Innate Immune System Pathway |
| MyD88 Cascade Initiated On Plasma Membrane Pathway | MyD88 Cascade Initiated On Plasma Membrane Pathway |
| MyD88 Dependent Cascade Initiated On Endosome Pathway | MyD88 Dependent Cascade Initiated On Endosome Pathway |
| MyD88-independent Cascade Pathway | MyD88-independent Cascade Pathway |
| MyD88:Mal Cascade Initiated On Plasma Membrane Pathway | MyD88:Mal Cascade Initiated On Plasma Membrane Pathway |
Diseases associated with S100 ELISA Kit
| Nervous System Diseases | publications with S100 and Nervous System Diseases |
| Brain Diseases | publications with S100 and Brain Diseases |
| Cardiovascular Diseases | publications with S100 and Cardiovascular Diseases |
| Brain Injuries | publications with S100 and Brain Injuries |
| Growth Disorders | publications with S100 and Growth Disorders |
| Inflammation | publications with S100 and Inflammation |
| Brain Ischemia | publications with S100 and Brain Ischemia |
| Disease Models, Animal | publications with S100 and Disease Models, Animal |
| Heart Diseases | publications with S100 and Heart Diseases |
| Necrosis | publications with S100 and Necrosis |
Organs/Tissues associated with S100 ELISA Kit
| Nerve | publications with S100 and Nerve |
| Brain | publications with S100 and Brain |
| Blood | publications with S100 and Blood |
| Heart | publications with S100 and Heart |
| Skin | publications with S100 and Skin |
| Eye | publications with S100 and Eye |
| Lung | publications with S100 and Lung |
| Spinal Cord | publications with S100 and Spinal Cord |
| Embryonic Tissue | publications with S100 and Embryonic Tissue |
| Connective Tissue | publications with S100 and Connective Tissue |
