KEY FEATURES
| Full Name | Prokineticin 2 |
|---|---|
| Synonym | PK-2; Bv8; MIT1; KAL4; Protein Bv8 Homolog |
| Assay Type | Competitive |
| Reactivity | Human |
| Range | 0.247-20ng/mL |
| Sensitivity | 0.091ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 50μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human PK2 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human PK2. During the reaction, Human PK2 in the sample or standard competes with a fixed amount of Human PK2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human PK2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human PK2 in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human PK2 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.92 | 1.73 | 8.46 | 0.86 | 1.82 | 8.78 |
| Standard deviation | 0.05 | 0.09 | 0.37 | 0.05 | 0.1 | 0.41 |
| CV(%) | 5.5 | 5.46 | 4.36 | 6.03 | 5.26 | 4.66 |
RECOVERY
The recovery of Human PK2 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-96 | 91 |
| EDTA plasma (n=8) | 90-105 | 96 |
| Cell culture media (n=8) | 92-105 | 97 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human PK2 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with PK2 ELISA Kits
| PROKR2 ELISA Kit | publications with PK2 and PROKR2 |
| GNRHR ELISA Kit | publications with PK2 and GNRHR |
| PROKR1 ELISA Kit | publications with PK2 and PROKR1 |
| TAC3 ELISA Kit | publications with PK2 and TAC3 |
| KISS1R ELISA Kit | publications with PK2 and KISS1R |
| TACR3 ELISA Kit | publications with PK2 and TACR3 |
| GNRH1 ELISA Kit | publications with PK2 and GNRH1 |
Pathways associated with PK2 ELISA Kit
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway | Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway |
| Disease Pathway | Disease Pathway |
| G Alpha (q) Signalling Events Pathway | G Alpha (q) Signalling Events Pathway |
| GPCR Downstream Signaling Pathway | GPCR Downstream Signaling Pathway |
| GPCR Ligand Binding Pathway | GPCR Ligand Binding Pathway |
| Gastrin-CREB Signalling Pathway Via PKC And MAPK | Gastrin-CREB Signalling Pathway Via PKC And MAPK |
| Metabolic Disorders Of Biological Oxidation Enzymes Pathway | Metabolic Disorders Of Biological Oxidation Enzymes Pathway |
| Peptide Ligand-binding Receptors Pathway | Peptide Ligand-binding Receptors Pathway |
| Signal Transduction Pathway | Signal Transduction Pathway |
Diseases associated with PK2 ELISA Kit
| Kallmann Syndrome | publications with PK2 and Kallmann Syndrome |
| Nervous System Diseases | publications with PK2 and Nervous System Diseases |
| Inflammation | publications with PK2 and Inflammation |
| Idiopathic Hypogonadotropic Hypogonadism | publications with PK2 and Idiopathic Hypogonadotropic Hypogonadism |
| Hyperalgesia | publications with PK2 and Hyperalgesia |
| Disease Models, Animal | publications with PK2 and Disease Models, Animal |
| Brain Diseases | publications with PK2 and Brain Diseases |
| Heart Diseases | publications with PK2 and Heart Diseases |
| Necrosis | publications with PK2 and Necrosis |
| Pancreatic Neoplasms | publications with PK2 and Pancreatic Neoplasms |
Organs/Tissues associated with PK2 ELISA Kit
| Brain | publications with PK2 and Brain |
| Vascular | publications with PK2 and Vascular |
| Bone Marrow | publications with PK2 and Bone Marrow |
| Testis | publications with PK2 and Testis |
| Placenta | publications with PK2 and Placenta |
| Intestine | publications with PK2 and Intestine |
| Kidney | publications with PK2 and Kidney |
| Spleen | publications with PK2 and Spleen |
