KEY FEATURES
| Full Name | Interleukin 10 |
|---|---|
| Synonym | CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor |
| Assay Type | Sandwich |
| Reactivity | Porcine |
| Range | 15.6-1000pg/mL |
| Sensitivity | 6.5pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Porcine IL-10 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine IL-10. You can calculate the concentration of Porcine IL-10 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Porcine IL-10 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 49.13 | 115.2 | 418.05 | 52.32 | 124.11 | 419.32 |
| Standard deviation | 3.02 | 4.64 | 19.73 | 2.9 | 7.26 | 14.26 |
| CV(%) | 6.14 | 4.03 | 4.72 | 5.54 | 5.85 | 3.4 |
RECOVERY
The recovery of Porcine IL-10 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 96-109 | 102 |
| EDTA plasma (n=8) | 90-105 | 96 |
| Cell culture media (n=8) | 87-98 | 92 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Porcine IL-10 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with IL10 ELISA Kits
| TNF ELISA Kit | publications with IL10 and TNF |
| CD4 ELISA Kit | publications with IL10 and CD4 |
| IL6 ELISA Kit | publications with IL10 and IL6 |
| IL4 ELISA Kit | publications with IL10 and IL4 |
| IL2 ELISA Kit | publications with IL10 and IL2 |
| NOS2 ELISA Kit | publications with IL10 and NOS2 |
| IL10RB ELISA Kit | publications with IL10 and IL10RB |
| IL10RA ELISA Kit | publications with IL10 and IL10RA |
| DIF ELISA Kit | publications with IL10 and DIF |
Pathways associated with IL10 ELISA Kit
| African Trypanosomiasis Pathway | African Trypanosomiasis Pathway |
| Allograft Rejection Pathway | Allograft Rejection Pathway |
| Amoebiasis Pathway | Amoebiasis Pathway |
| Asthma Pathway | Asthma Pathway |
| Autoimmune Thyroid Disease Pathway | Autoimmune Thyroid Disease Pathway |
Diseases associated with IL10 ELISA Kit
| Necrosis | publications with IL10 and Necrosis |
| Inflammation | publications with IL10 and Inflammation |
| Sepsis | publications with IL10 and Sepsis |
| Colitis | publications with IL10 and Colitis |
| Genetic Predisposition to Disease | publications with IL10 and Genetic Predisposition to Disease |
| Hypersensitivity, Immediate | publications with IL10 and Hypersensitivity, Immediate |
| Inflammatory Bowel Diseases | publications with IL10 and Inflammatory Bowel Diseases |
| Arthritis, Rheumatoid | publications with IL10 and Arthritis, Rheumatoid |
| Kidney Diseases | publications with IL10 and Kidney Diseases |
| HIV Infections | publications with IL10 and HIV Infections |
Organs/Tissues associated with IL10 ELISA Kit
| Blood | publications with IL10 and Blood |
| Spleen | publications with IL10 and Spleen |
| Lung | publications with IL10 and Lung |
| Connective Tissue | publications with IL10 and Connective Tissue |
| Uncharacterized Tissue | publications with IL10 and Uncharacterized Tissue |
