KEY FEATURES
| Full Name | Caspase 9 |
|---|---|
| Synonym | APAF3; ICE-LAP6; MCH6; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 9; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6 |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.07ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse CASP9 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CASP9. You can calculate the concentration of Mouse CASP9 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse CASP9 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.48 | 1.52 | 4.7 | 0.43 | 1.65 | 5.05 |
| Standard deviation | 0.03 | 0.08 | 0.24 | 0.03 | 0.09 | 0.21 |
| CV(%) | 6.48 | 5.39 | 5.09 | 5.92 | 5.55 | 4.19 |
RECOVERY
The recovery of Mouse CASP9 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-100 | 92 |
| EDTA plasma (n=8) | 86-97 | 91 |
| Cell culture media (n=8) | 91-105 | 97 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse CASP9 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CASP9 ELISA Kits
| CASP3 ELISA Kit | publications with CASP9 and CASP3 |
| BCL2L1 ELISA Kit | publications with CASP9 and BCL2L1 |
| APAF1 ELISA Kit | publications with CASP9 and APAF1 |
| XIAP ELISA Kit | publications with CASP9 and XIAP |
| DIABLO ELISA Kit | publications with CASP9 and DIABLO |
| CASP1 ELISA Kit | publications with CASP9 and CASP1 |
| AKT1 ELISA Kit | publications with CASP9 and AKT1 |
| BIRC3 ELISA Kit | publications with CASP9 and BIRC3 |
| BIRC2 ELISA Kit | publications with CASP9 and BIRC2 |
| DCC ELISA Kit | publications with CASP9 and DCC |
Pathways associated with CASP9 ELISA Kit
| AGE/RAGE Pathway | AGE/RAGE Pathway |
| AKT Phosphorylates Targets In The Cytosol Pathway | AKT Phosphorylates Targets In The Cytosol Pathway |
| Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway | Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway |
| Adaptive Immune System Pathway | Adaptive Immune System Pathway |
| Alzheimer's Disease Pathway | Alzheimer's Disease Pathway |
| Alzheimers Disease Pathway | Alzheimers Disease Pathway |
| Amyotrophic Lateral Sclerosis (ALS) Pathway | Amyotrophic Lateral Sclerosis (ALS) Pathway |
| Apoptosis Pathway | Apoptosis Pathway |
Diseases associated with CASP9 ELISA Kit
| Neoplasms | publications with CASP9 and Neoplasms |
| Necrosis | publications with CASP9 and Necrosis |
| Drug Toxicity | publications with CASP9 and Drug Toxicity |
| Nervous System Diseases | publications with CASP9 and Nervous System Diseases |
| Gastrointestinal Diseases | publications with CASP9 and Gastrointestinal Diseases |
| Brain Diseases | publications with CASP9 and Brain Diseases |
| Breast Neoplasms | publications with CASP9 and Breast Neoplasms |
| Liver Diseases | publications with CASP9 and Liver Diseases |
| Inflammation | publications with CASP9 and Inflammation |
| Lung Neoplasms | publications with CASP9 and Lung Neoplasms |
Organs/Tissues associated with CASP9 ELISA Kit
| Blood | publications with CASP9 and Blood |
| Brain | publications with CASP9 and Brain |
| Liver | publications with CASP9 and Liver |
| Lung | publications with CASP9 and Lung |
| Muscle | publications with CASP9 and Muscle |
| Vascular | publications with CASP9 and Vascular |
| Heart | publications with CASP9 and Heart |
| Prostate | publications with CASP9 and Prostate |
| Kidney | publications with CASP9 and Kidney |
| Bone | publications with CASP9 and Bone |
