KEY FEATURES
| Full Name | Inhibin Beta B |
|---|---|
| Synonym | INH-BB; Activin AB Beta Polypeptide |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 15.6-1000pg/mL |
| Sensitivity | 6pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human INHbB in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INHbB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INHbB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INHbB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INHbB. You can calculate the concentration of Human INHbB in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human INHbB ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 53.07 | 151.82 | 376.7 | 56.43 | 152.95 | 399.97 |
| Standard deviation | 3.43 | 7.01 | 17.06 | 3.5 | 7.37 | 12.64 |
| CV(%) | 6.47 | 4.62 | 4.53 | 6.21 | 4.82 | 3.16 |
RECOVERY
The recovery of Human INHbB spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-95 | 90 |
| EDTA plasma (n=8) | 92-105 | 97 |
| Cell culture media (n=8) | 88-102 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human INHbB in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with INH ELISA Kits
| INHBA ELISA Kit | publications with INH and INHBA |
| INHA ELISA Kit | publications with INH and INHA |
| FST ELISA Kit | publications with INH and FST |
| FSHB ELISA Kit | publications with INH and FSHB |
| INHBC ELISA Kit | publications with INH and INHBC |
| ACVR1B ELISA Kit | publications with INH and ACVR1B |
| INHBE ELISA Kit | publications with INH and INHBE |
| ACVR2A ELISA Kit | publications with INH and ACVR2A |
Pathways associated with INH ELISA Kit
| AGE/RAGE Pathway | AGE/RAGE Pathway |
| Antagonism Of Activin By Follistatin Pathway | Antagonism Of Activin By Follistatin Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Glycoprotein Hormones Pathway | Glycoprotein Hormones Pathway |
| Metabolism Of Proteins Pathway | Metabolism Of Proteins Pathway |
| Peptide Hormone Biosynthesis Pathway | Peptide Hormone Biosynthesis Pathway |
| Peptide Hormone Metabolism Pathway | Peptide Hormone Metabolism Pathway |
| Signal Transduction Pathway | Signal Transduction Pathway |
| Signaling By Activin Pathway | Signaling By Activin Pathway |
Diseases associated with INH ELISA Kit
| Ovarian Diseases | publications with INH and Ovarian Diseases |
| Infertility, Male | publications with INH and Infertility, Male |
| Adenocarcinoma | publications with INH and Adenocarcinoma |
| Ovarian Neoplasms | publications with INH and Ovarian Neoplasms |
| Prostatic Neoplasms | publications with INH and Prostatic Neoplasms |
| Weight Loss | publications with INH and Weight Loss |
| Disease Models, Animal | publications with INH and Disease Models, Animal |
| Breast Neoplasms | publications with INH and Breast Neoplasms |
| Neoplasm Metastasis | publications with INH and Neoplasm Metastasis |
| Congenital Abnormalities | publications with INH and Congenital Abnormalities |
Organs/Tissues associated with INH ELISA Kit
| Testis | publications with INH and Testis |
| Brain | publications with INH and Brain |
| Uterus | publications with INH and Uterus |
| Liver | publications with INH and Liver |
| Pancreas | publications with INH and Pancreas |
| Prostate | publications with INH and Prostate |
| Kidney | publications with INH and Kidney |
| Eye | publications with INH and Eye |
| Nerve | publications with INH and Nerve |
| Lung | publications with INH and Lung |
