KEY FEATURES
| Full Name | Arrestin Beta 2 |
|---|---|
| Synonym | ARB2; ARR2; ARR-B2; Arrestin 3 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 15.62-1000pg/mL |
| Sensitivity | 6.4pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ARRb2 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ARRb2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARRb2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARRb2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ARRb2. You can calculate the concentration of Human ARRb2 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ARRb2 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 48.2 | 122.11 | 481.68 | 49.55 | 127.64 | 518.15 |
| Standard deviation | 2.85 | 5.43 | 23.6 | 3.43 | 7.63 | 19.43 |
| CV(%) | 5.91 | 4.45 | 4.9 | 6.92 | 5.98 | 3.75 |
RECOVERY
The recovery of Human ARRb2 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-106 | 99 |
| EDTA plasma (n=8) | 89-102 | 96 |
| Cell culture media (n=8) | 95-108 | 100 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ARRb2 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with ARRB2 ELISA Kits
| ADRB2 ELISA Kit | publications with ARRB2 and ADRB2 |
Pathways associated with ARRB2 ELISA Kit
| ALK1 Signaling Events Pathway | ALK1 Signaling Events Pathway |
| Activated NOTCH1 Transmits Signal To The Nucleus Pathway | Activated NOTCH1 Transmits Signal To The Nucleus Pathway |
| Activation Of SMO Pathway | Activation Of SMO Pathway |
| Arf6 Signaling Events Pathway | Arf6 Signaling Events Pathway |
| Atypical NF-kappaB Pathway | Atypical NF-kappaB Pathway |
| CXCR4-mediated Signaling Events Pathway | CXCR4-mediated Signaling Events Pathway |
| Calcium Regulation In The Cardiac Cell Pathway | Calcium Regulation In The Cardiac Cell Pathway |
| Chemokine Signaling Pathway | Chemokine Signaling Pathway |
| Corticotropin-releasing Hormone Pathway | Corticotropin-releasing Hormone Pathway |
Diseases associated with ARRB2 ELISA Kit
| Inflammation | publications with ARRB2 and Inflammation |
| Brain Diseases | publications with ARRB2 and Brain Diseases |
| Poisoning | publications with ARRB2 and Poisoning |
| Amphetamine-Related Disorders | publications with ARRB2 and Amphetamine-Related Disorders |
| Drug-Related Side Effects and Adverse Reactions | publications with ARRB2 and Drug-Related Side Effects and Adverse Reactions |
| Liver Diseases | publications with ARRB2 and Liver Diseases |
| Hyperalgesia | publications with ARRB2 and Hyperalgesia |
| Myocardial Ischemia | publications with ARRB2 and Myocardial Ischemia |
| Movement Disorders | publications with ARRB2 and Movement Disorders |
| Kidney Diseases | publications with ARRB2 and Kidney Diseases |
Organs/Tissues associated with ARRB2 ELISA Kit
| Brain | publications with ARRB2 and Brain |
| Blood | publications with ARRB2 and Blood |
| Bone | publications with ARRB2 and Bone |
| Liver | publications with ARRB2 and Liver |
| Kidney | publications with ARRB2 and Kidney |
| Lung | publications with ARRB2 and Lung |
| Eye | publications with ARRB2 and Eye |
| Bone Marrow | publications with ARRB2 and Bone Marrow |
| Nerve | publications with ARRB2 and Nerve |
| Skin | publications with ARRB2 and Skin |
