KEY FEATURES
| Full Name | Polymeric Immunoglobulin Receptor |
|---|---|
| Synonym | SC; Secretory Component; Hepatocellular carcinoma-associated protein TB6 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 62.5-4000pg/mL |
| Sensitivity | 23.7pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human PIGR in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIGR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PIGR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIGR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PIGR. You can calculate the concentration of Human PIGR in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human PIGR ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 194.22 | 553.06 | 1619.53 | 181.63 | 557.3 | 1597.76 |
| Standard deviation | 12.35 | 25.5 | 79.2 | 10.84 | 32.99 | 70.94 |
| CV(%) | 6.36 | 4.61 | 4.89 | 5.97 | 5.92 | 4.44 |
RECOVERY
The recovery of Human PIGR spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-104 | 99 |
| EDTA plasma (n=8) | 91-105 | 99 |
| Cell culture media (n=8) | 87-102 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human PIGR in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with PIGR ELISA Kits
| PIGA ELISA Kit | publications with PIGR and PIGA |
| STAT6 ELISA Kit | publications with PIGR and STAT6 |
| RAB3B ELISA Kit | publications with PIGR and RAB3B |
Pathways associated with PIGR ELISA Kit
| IL4-mediated Signaling Events Pathway | IL4-mediated Signaling Events Pathway |
| Integrated Breast Cancer Pathway | Integrated Breast Cancer Pathway |
| Intestinal Immune Network For IgA Production Pathway | Intestinal Immune Network For IgA Production Pathway |
Diseases associated with PIGR ELISA Kit
| Inflammation | publications with PIGR and Inflammation |
| Neoplasms | publications with PIGR and Neoplasms |
| Necrosis | publications with PIGR and Necrosis |
| Kidney Diseases | publications with PIGR and Kidney Diseases |
| Adenocarcinoma | publications with PIGR and Adenocarcinoma |
| Liver Diseases | publications with PIGR and Liver Diseases |
| Glomerulonephritis, IGA | publications with PIGR and Glomerulonephritis, IGA |
| Cell Transformation, Neoplastic | publications with PIGR and Cell Transformation, Neoplastic |
| Cholestasis | publications with PIGR and Cholestasis |
| Drug Toxicity | publications with PIGR and Drug Toxicity |
Organs/Tissues associated with PIGR ELISA Kit
| Intestine | publications with PIGR and Intestine |
| Kidney | publications with PIGR and Kidney |
| Liver | publications with PIGR and Liver |
| Lung | publications with PIGR and Lung |
| Mammary Gland | publications with PIGR and Mammary Gland |
| Uterus | publications with PIGR and Uterus |
| Muscle | publications with PIGR and Muscle |
| Brain | publications with PIGR and Brain |
| Stomach | publications with PIGR and Stomach |
| Skin | publications with PIGR and Skin |
