Cyanine7 Labeling Kit-EnkiLife

The EnkiLife Cyanine7 labeling kit includes multiple components for labeling antibody proteins, such as high - performance Cyanine7 fluorescent dye, ultrafiltration centrifuge tubes for purification, and labeling buffers. It enables easy and reliable labeling of antibodies and proteins.

Features

Fast: Labeling takes only about 30 minutes, balancing labeling effectiveness and efficiency.
Convenient: Each dye reagent is pre - optimized for corresponding antibody amounts, eliminating complex calculations. The solid dye form ensures batch stability, and following the steps yields good results.
Flexible: In the large - scale kit, the dye is pre - portioned for consistent batch performance, making it convenient for multiple labeling uses.
Outstanding labeling performance: The optimized labeling buffer ensures relatively fixed dye labeling sites, enhancing the homogeneity of labeled antibodies.
Amine - specific labeling: The NHS ester on the fluorescent dye efficiently labels antibodies and other purified proteins at primary amines.
Labeled proteins: This kit is mainly designed for IgG antibody labeling. Proteins similar in size and properties to IgG antibodies can also be labeled.

Dye Introduction

Cyanine7 is a near - infrared fluorescent dye commonly used in small animal in - vivo imaging. Its fluorescence is invisible to the naked eye and requires a near - infrared filter for microscopy. Many fluorescence microscopes (including confocal microscopes) lack this filter, but flow cytometers often have a Cyanine7 filter set. As a commonly used fluorescent dye, Cyanine7 can label nucleic acids, proteins, antibodies, peptides, etc., making it one of the most common small - animal imaging agents.

Properties of Cyanine7 Dye

Similar Dyes: Fluor750, IRdye 800CW, DyLight 755, ICG

Excitation Light Source: 750 nm ± 5 nm

Excitation Wavelength: 750 nm

Emission Wavelength: 773 nm

Molar Extinction Coefficient (L/(mol・cm)): 200,000

Purity: > 95%

Solubility: Soluble in organic solvents like methanol and DMF, and also in water

Reactive Group: NHS ester, which reacts with primary amine groups

Chemical Structure:

Cyanine7 Dye Spectrum

Kit Specifications

Product composition	Content of components in different specifications			Storage temperature
Product composition	40 μg antibody	200 μg antibody	2 mg antibody	Storage temperature
Cyanine7	1 tube	5 tubes	5 tubes	-20 °C after opening, away from light
Ultrafiltration Tube* ,50K MWCO	1 set **	1 set **	1 set **	RT
Labeling Buffer S	10 mL	10 mL	10 mL	2-8 °C
1× PBS (pH 7.4)	10 mL	10 mL	10 mL	2-8 °C
DMF	100 μL	100 μL	100 μL	2-8 °C, away from light
Labeled protein storage solution	200 μL	1mL	5mL	2-8 °C
Recommended amount of labeled antibody	Each tube is labeled with 20-40 μg, and the best is 20 μg	Each tube is labeled with 20-40 μg, and the best is 20 μg	Each tube is labeled with 100-400 μg, and the best is 200 μg

Application Examples of Cyanine7 Dye

lCyanine7 labeled anti-EGFR antibodies for in - vivo imaging → injected into colon cancer bearing mice via tail vein → 48 hours later, fluorescence intensity in tumor areas increased eightfold compared to normal tissues.

lLiquid - phase chip multiplexed detection (Microsphere 1: coated with IL-6 antibodies + Cyanine7-labeled secondary antibodies; Microsphere 2: coated with TNFα antibodies + QD705-labeled secondary antibodies).

lInvitro labeling of T cells (CD8⁺-Cyanine7) → adoptive transfer into melanoma models → real-time observation of T cell tumor infiltration revealed that immune checkpoint inhibitors (PD-1 antibodies) facilitated a threefold faster CD8⁺ T cell tumor infiltration.

lCyanine7 can be tandem - linked with R-PE to form PE/Cyanine7, which is used for labeling antibodies for multicolor flow cytometry phenotypic analysis.

Cyanine7 - Antibody Labeling Principle

Within a certain pH range, the activated group of the fluorescent dye specifically reacts with primary amino groups on antibody proteins to form stable amide bonds, thereby conjugating the dye to the antibody protein.