Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of 3,5-dinitrosalicylohydrazide content in Food samples.
Key features
Assay Range: 0.02 ppb~0.54 ppb
Sensitivity: 0.02 ppb (ng/mL)
Species reactivity: Universal
Assay time: 75min
Result type: Quantitative
Category: Residues&contaminants
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 90±30%
Specificity:
3,5-dinitrosalicylohydrazide | 100% | ||||
Nifursol, Furacilin and its metabolites, Furazolidone and its metabolites, Furaltadone and its metabolites, Nitrofurantoin and its metabolites | ＜0.1% |
Detection limits:
Muscle, Aquatic products | 0.25 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. 3,5-dinitrosalicylohydrazide in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for 3,5-dinitrosalicylohydrazide specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of 3,5-dinitrosalicylohydrazide it contains, and the residual amount of 3,5-dinitrosalicylohydrazide in the sample can be obtained by comparing with the standard curve.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Standard | 5 x 1.5 mL | ||||
HRP Conjugate | 1 x 12 mL | ||||
Antibody | 1 x 7 mL | ||||
50 mM 2-Nitrobenzaldehyde | 1 x 10 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Reconstitution Buffer | 1 x 60 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
Standard curve reference
Standard | 1 | 2 | 3 | 4 | 5 | |||
Concentration (ppb=ng/mL=ng/g) | 0 | 0.02 | 0.06 | 0.18 | 0.54 | |||
OD Standard | 2.224 | 1.694 | 1.215 | 0.635 | 0.279 |
