Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Aflatoxin M1 content in Food samples.
Key features
Assay Range: 0.005 ppb~0.405 ppb
Sensitivity: 0.005 ppb (ng/mL)
Species reactivity: Universal
Assay time: 55min
Result type: Quantitative
Category: Mycotoxins
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 100%±30%
Specificity:
Aflatoxin M1 | 100% | ||||
Aflatoxin M2 | ＜1.0% | ||||
Aflatoxin G1 | 20% | ||||
Aflatoxin G2 | 2% | ||||
Aflatoxin B1 | 40% | ||||
Aflatoxin B2 | 3% |
Detection limits:
Raw milk, pasteurized milk, sterilized milk, reconstituted milk, modified milk | 0.01 ppb | ||||
Cheese, cream, yoghurt | 0.25 ppb | ||||
Milk powder | 0.06 ppb | ||||
Whey protein powder, demineralized whey powder, milk protein powder, condensed milk | 0.10 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. Aflatoxin M1 in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for Aflatoxin M1 specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of Aflatoxin M1 it contains, and the residual amount of Aflatoxin M1 in the sample can be obtained by comparing with the standard curve.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Standard | 6 x 1.5 mL | ||||
HRP Conjugate concentrate (11×) | 1 x 1 mL | ||||
HRP Conjugate diluent | 1 x 10 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Sample dilution | 1 x 50 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
Standard curve reference
Standard | 1 | 2 | 3 | 4 | 5 | 6 | |||
Concentration (ppb=ng/mL=ng/g) | 0 | 0.005 | 0.015 | 0.045 | 0.135 | 0.405 | |||
OD Standard | 2.156 | 1.768 | 1.266 | 0.641 | 0.254 | 0.084 |
