BCA Protein Assay Kit (BCA Method)

BCA Protein Assay Kit (BCA Method)

Cat: BC00006
Size1:200T Price1:$120
Size2:600T Price2:$180
Range:0.0625-1.5mg/mL
Sensitivity:0.002438mg/mL
Detection Method:Colorimetric;Quantitative
Detection Instrument:Microplate reader(540-595 nm,optimum wavelength:562 nm)
Sample Type:serum, plasma, Urine, Animal and Plant Tissues, Culture Medium, cells
Category: Metabolism Kits Tags: ,

Product Introduction

This kit is a kit commonly used to determine the protein content in biological samples. The BCA method (Bicinchonininc Acid Method ) is a method for determining protein concentration.

 

Principle                        

The BCA (Bicinchonininc Acid) protein content detection method is based on the ability of protein to reduce Cu2+ to Cu+ under alkaline conditions. Cu+ and BCA reagent can form a purple complex. The product has a characteristic absorption peak at 562 nm. The protein concentration of the sample can be quantitatively detected by the change in absorbance value.

Components

Serial number

Components

Size(200T)

Size(600T)

Storage

Reagent 1

BCA Reagent A

40 ml

120 ml

-20

Reagent 2

BCA Reagent B

1.2 ml

1.2 ml*3 vials

-20

Reagent 3

BSA Protein Standard

20 mg

20 mg*3 vials

-20; after being prepared into solution, store at -20.

Reagent 4

Protein Standard Preparation Solution

1 ml

1 ml * 3 vials

-20

Consumable 1

Microplate(96 wells)

2 plates

6 plates

RT

Consumable 2

Plate Sealer

4 pieces

12 pieces

RT


Storage

The unopened kit can be stored at -20 for 12 months.


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