Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Beta-adrenergic agonists content in Food samples.
Key features
Assay Range: 0.05 ppb~4.05 ppb
Sensitivity: 0.05 ppb (ng/mL)
Species reactivity: Universal
Assay time: 45min
Result type: Quantitative
Category: Residues&contaminants
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 90%±30%
Specificity:
Clenbuterol | 100% | ||||
Bambuterol | 175% | ||||
Mabuterol | 130% | ||||
Brombuterol | 88% | ||||
Clenproperol | 75% | ||||
Cimbuterol | 41% | ||||
Cimaterol | 20% |
Detection limits:
Pig urine | 0.4 ppb | ||||
Cow urine, pork, beef, lamb | 0.5 ppb | ||||
Feed | 10.0 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. Beta-adrenergic agonists in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for Beta-adrenergic agonists specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of Beta-adrenergic agonists it contains, and the residual amount of Beta-adrenergic agonists in the sample can be obtained by comparing with the standard curve.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Standard | 6 x 1.5 mL | ||||
HRP Conjugate | 1 x 6 mL | ||||
Antibody | 1 x 6 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Feed diluent | 1 x 30 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
Standard curve reference
Standard | 1 | 2 | 3 | 4 | 5 | 6 | |||
Concentration (ppb=ng/mL=ng/g) | 0 | 0.05 | 0.15 | 0.45 | 1.35 | 4.05 | |||
OD Standard | 1.916 | 1.652 | 1.150 | 0.626 | 0.213 | 0.062 |
