Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Beta-lactam antibiotic content in Food samples.
Key features
Assay Range: 0.2 ppb~16.2 ppb
Sensitivity: 0.2 ppb (ng/mL)
Species reactivity: Universal
Assay time: 75min
Result type: Quantitative
Category: Residues&contaminants
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 90%±30%
Specificity:
Ampicillin | 110% | ||||
Amoxicillin | 100% | ||||
Cloxacillin | 110% | ||||
Oxacillin | 85% | ||||
Penicillin | 500% | ||||
Dicloxacillin | 210% | ||||
Cefoperazone | 250% | ||||
Cefalonium | 400% | ||||
Nafcillin | 30% | ||||
Cefotaxime | 30% | ||||
Cephapiriin | 30% | ||||
Cephacetrile | 5% | ||||
Ceftizoxime | 5% |
Detection limits:
Raw milk, reconstituted milk, sterilized milk, pasteurized milk | 1 ppb | ||||
Pork, beef, chicken, lamb, duck | 10 ppb | ||||
Egg, pigeon egg | 2 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. Beta-lactam antibiotic in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for Beta-lactam antibiotic specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of Beta-lactam antibiotic it contains, and the residual amount of Beta-lactam antibiotic in the sample can be obtained by comparing with the standard curve.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Standard | 6 x 1.5 mL | ||||
HRP Conjugate | 1 x 12 mL | ||||
Antibody | 1 x 7 mL | ||||
Concentrated sample dilution (5×) | 1 x 20 mL | ||||
Dairy extract | 1 x 50 mL | ||||
Dairy dilution | 1 x 10 mL | ||||
Egg dilution | 1 x 50 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
Standard curve reference
Standard | 1 | 2 | 3 | 4 | 5 | 6 | |||
Concentration (ppb=ng/mL=ng/g) | 0 | 0.2 | 0.6 | 1.8 | 5.4 | 16.2 | |||
OD Standard | 2.036 | 1.675 | 1.222 | 0.702 | 0.302 | 0.122 |
