Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Biotin content in Food samples.
Key features
Assay Range: 0.08~0.72 μg/100 g(mL)
Sensitivity: 0.8 ppb (ng/mL)
Species reactivity: Universal
Assay time: 44-48h
Result type: Quantitative
Category: Vitamins
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 90~105%
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
Biotin is an essential nutrient for the growth of Lactobacillus plantarum. Within a certain range of biotin concentration, the growth turbidity of this strain shows a linear positive correlation with the biotin concentration.
VH is extracted from the sample and the extract is diluted. The VH assay-medium and the diluted extract are pipetted into the wells of a microtiter plate which is coated with Lactobacillus plantarum. The incubation is done in the dark at 37 °C (98.6 °F) for 44-48 h. The growth of Lactobacillus plantarum is dependent on the supply of VH. Following the addition of VH as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The intensity of metabolism or growth of Lactobacillus plantarum in relation to the extracted VH is measured as turbidity and compared to a standard curve. The measurement is done using microplate reader at 620 nm.
Materials supplied
Item | Quantity | ||||
Assay Plate (coated with Lactobacillus plantarum) | 96T | ||||
VH Standard (solid) | 3 bottles | ||||
VH Assay Medium (solid) | 3 bottles | ||||
Sterile Water | 3 x 30 mL | ||||
Plate Sealer | 2 pieces | ||||
Instruction Manual | 1 piece |
