Brachyury (4T11) Rabbit Monoclonal Antibody

Summary

Production Name	Brachyury (4T11) Rabbit Monoclonal Antibody
Description	Recombinant rabbit monoclonal antibody
Host	Rabbit
Application	WB,IHC,ICC/IF,FC,IP
Reactivity	Human,Mouse,Rat

Performance

Conjugation	Unconjugated
Modification	Unmodified
Isotype	IgG
Clonality	Monoclonal
Form	Liquid
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification	Affinity purification

Immunogen

Gene Name	TBXT
Alternative Names	Brachyury homolog; Bry; Protein T; SAVA; TFT; T-box transcription factor T; TBXT; Brachyury protein;
Gene ID	6862
SwissProt ID	O15178

Application

Dilution Ratio	WB 1:500-1:2000,IHC 1:1000-1:5000,ICC/IF 1:500-1:1000,FC 1:200-1:500,IP 1:20-1:50
Molecular Weight	47kDa

Background

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.

Research Area

Stem Cells; Lineage Markers; Mesoderm; Hematopoietic Progenitors; Intracellular Molecules; Cardiovascular; Heart; Cardiogenesis; Transcription factors/regulators; Developmental Biology; Lineage specification; Mesoderm