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Ham's F-10 (Without Phenol Red) Complete Medium

Ham's F-10 (Without Phenol Red) Complete Medium

Cat. No.: CMC0047
Size:500mL price:$128 Category: Complete Media Tags:

Ham's F-10 Nutrient Mixture is a culture medium designed by Ham in 1963 for serum-free culture of CHO cells. Ham's F-10 contains rich nutrients and is suitable for the culture of human diploid cells, cell cloning culture under low serum conditions, normal culture of mammalian cells, chromosome analysis of leukocytes, and primary cell culture of rats, rabbits, chickens and other animals.

 

Phenol red is used as a pH indicator in culture media to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, the culture medium turns purple. It turns red at pH 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium that does not contain phenol red. Phenol red can interfere with detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas can interfere with sodium-potassium balance.

 

Details

Form

Liquid

Size

500mL

Components

Ham's F-10 (Without Phenol Red)(CMB0088)+10% Nutrient+1% Supplement

Concentration

Ready-to-use

Bacteria Detection

Negative

Fungi Detection

Negative

Mycoplasma Detection

Negative

Endotoxin Content（EU/mL）

＜3

L-Glutamine

1mM

NaHCO3

1200mg/L

D-Glucose

1100mg/L

Sodium Pyruvate

Without

HEPES

Without

Phenol Red

Without

Balanced Salt Solution

Without

Nucleoside

Without

NEAA

Without

D-Galactose

Without

Transportation

2-8°C

Storage

Store at 2-8°C away from light

Shelf Life

6 months

 

Precautions

1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;

2. To maintain the best use effect of this product, please do not perform freeze-thaw treatment;

3. This product is only used for research or further research, not for diagnosis and treatment.

4. It is not suitable for long time storage at room temperature.

5. This product is a ready-to-use medium. If there is no special need, don't add serum, penicillin and streptomycin. It can be used directly.

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