Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Chloramphenicol content in Food samples.
Key features
Assay Range: 0.02 ppb~0.72 ppb
Sensitivity: 0.02 ppb (ng/mL)
Species reactivity: Universal
Assay time: 30min
Result type: Quantitative
Category: Residues&contaminants
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 90%±30%
Specificity:
Chloramphenicol | 100% | ||||
Chloramphenicol succinate | 51% | ||||
Thiamphenicol, Florfenicol, Florfenicol amine | ＜1% |
Detection limits:
Gentamicin Sulfate Powder, Erythromycin Thiocyanate Powder, Methenamine-Vitamin B Complex Powder, Vitamin AD Complex Powder, Apramycin Sulfate-Sulbactam Sodium Complex Powder, Apramycin Sulfate-Kanamycin Sulfate Complex Powder, Sodium Bicarbonate Powder, Astragalus Polysaccharide Oral Liquid | 1 ppb | ||||
Lincomycin Hydrochloride-Ampicillin Sodium-Sulbactam Sodium Complex Powder, Maltosaccharide Oligomer Powder, Astragalus Polysaccharide-Ranitidine Hydrochloride Complex Powder | 1 ppb | ||||
Astragalus Polysaccharide-Aminopyrine Complex Powder, Zeolite Powder-Montmorillonite Complex Powder | 1 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. Chloramphenicol in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for Chloramphenicol specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of Chloramphenicol it contains, and the residual amount of Chloramphenicol in the sample can be obtained by comparing with the standard curve.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Standard | 6 x 1.5 mL | ||||
High concentration standard (10 ppb) | 1 x 1 mL | ||||
HRP Conjugate concentrate (5×) | 1 x 2 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Concentrated Sample dilution (10×) | 1 x 50 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
Standard curve reference
Standard | 1 | 2 | 3 | 4 | 5 | 6 | |||
Concentration (ppb=ng/mL=ng/g) | 0 | 0.02 | 0.06 | 0.18 | 0.36 | 0.72 | |||
OD Standard | 2.087 | 1.841 | 1.317 | 0.663 | 0.382 | 0.246 |
