Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Cyanocobalamin content in Food samples.
Key features
Assay Range: 0.2 ppb~16.2 ppb
Sensitivity: 0.2 ppb (ng/mL)
Species reactivity: Universal
Assay time: 45min
Result type: Quantitative
Category: Vitamins
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 90%±30%
Specificity:
VB12 | 100% |
Detection limits:
Milk powder | 10 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. Cyanocobalamin in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for Cyanocobalamin specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of Cyanocobalamin it contains, and the residual amount of Cyanocobalamin in the sample can be obtained by comparing with the standard curve.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Standard | 6 x 1.5 mL | ||||
HRP Conjugate | 1 x 7 mL | ||||
Concentrated Standard (1 ppm) | 1 x 1 mL | ||||
Antibody | 1 x 7 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Concentrated sample diluent (2×) | 1 x 50 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
Standard curve reference
Standard | 1 | 2 | 3 | 4 | 5 | 6 | |||
Concentration (ppb=ng/mL=ng/g) | 0 | 0.2 | 0.6 | 1.8 | 5.4 | 16.2 | |||
OD Standard | 1.902 | 1.466 | 1.077 | 0.610 | 0.192 | 0.057 |
