KEY FEATURES
| Full Name | Actin Alpha 1, Cardiac Muscle |
|---|---|
| Synonym | ACTC; CMD1R; CMD1-R; ACT-a1 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ACTC1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACTC1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACTC1. You can calculate the concentration of Human ACTC1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ACTC1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.51 | 1.42 | 4.11 | 0.54 | 1.47 | 4.1 |
| Standard deviation | 0.03 | 0.08 | 0.21 | 0.03 | 0.08 | 0.18 |
| CV(%) | 6.42 | 5.47 | 5.19 | 6.35 | 5.17 | 4.34 |
RECOVERY
The recovery of Human ACTC1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-103 | 95 |
| EDTA plasma (n=8) | 89-100 | 95 |
| Cell culture media (n=8) | 90-101 | 95 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ACTC1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with ACTC1 ELISA Kits
| MYH7 ELISA Kit | publications with ACTC1 and MYH7 |
| MYH6 ELISA Kit | publications with ACTC1 and MYH6 |
| ACTA1 ELISA Kit | publications with ACTC1 and ACTA1 |
Pathways associated with ACTC1 ELISA Kit
| Cardiac Progenitor Differentiation Pathway | Cardiac Progenitor Differentiation Pathway |
| Cardiac Muscle Contraction Pathway | Cardiac Muscle Contraction Pathway |
| Dilated Cardiomyopathy Pathway | Dilated Cardiomyopathy Pathway |
| Hypertrophic Cardiomyopathy (HCM) Pathway | Hypertrophic Cardiomyopathy (HCM) Pathway |
| Myometrial Relaxation And Contraction Pathways | Myometrial Relaxation And Contraction Pathways |
| Striated Muscle Contraction Pathway | Striated Muscle Contraction Pathway |
Diseases associated with ACTC1 ELISA Kit
| Cardiovascular Diseases | publications with ACTC1 and Cardiovascular Diseases |
| Cardiomyopathy, Dilated | publications with ACTC1 and Cardiomyopathy, Dilated |
| Cardiomyopathy, Familial Hypertrophic, 1 | publications with ACTC1 and Cardiomyopathy, Familial Hypertrophic, 1 |
| Neoplasms | publications with ACTC1 and Neoplasms |
| Hypertrophy | publications with ACTC1 and Hypertrophy |
| Cardiomyopathy, Familial Hypertrophic, 11 | publications with ACTC1 and Cardiomyopathy, Familial Hypertrophic, 11 |
| Disease Models, Animal | publications with ACTC1 and Disease Models, Animal |
| Lung Diseases | publications with ACTC1 and Lung Diseases |
| Neoplasms, Experimental | publications with ACTC1 and Neoplasms, Experimental |
| Lung Neoplasms | publications with ACTC1 and Lung Neoplasms |
Organs/Tissues associated with ACTC1 ELISA Kit
| Muscle | publications with ACTC1 and Muscle |
| Heart | publications with ACTC1 and Heart |
| Vascular | publications with ACTC1 and Vascular |
| Lung | publications with ACTC1 and Lung |
| Embryonic Tissue | publications with ACTC1 and Embryonic Tissue |
| Bone | publications with ACTC1 and Bone |
| Prostate | publications with ACTC1 and Prostate |
