KEY FEATURES
| Full Name | Adenosine Deaminase |
|---|---|
| Synonym | Adenosine Aminohydrolase |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.07ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ADA in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADA. You can calculate the concentration of Human ADA in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ADA ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.54 | 1.27 | 4.45 | 0.49 | 1.25 | 4.24 |
| Standard deviation | 0.03 | 0.07 | 0.23 | 0.03 | 0.07 | 0.13 |
| CV(%) | 5.1 | 5.86 | 5.2 | 5.43 | 5.62 | 3.1 |
RECOVERY
The recovery of Human ADA spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-102 | 94 |
| EDTA plasma (n=8) | 93-107 | 99 |
| Cell culture media (n=8) | 95-108 | 100 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ADA in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with ADA ELISA Kits
| PNP ELISA Kit | publications with ADA and PNP |
| ADORA1 ELISA Kit | publications with ADA and ADORA1 |
| DPP4 ELISA Kit | publications with ADA and DPP4 |
| DCK ELISA Kit | publications with ADA and DCK |
| MYB ELISA Kit | publications with ADA and MYB |
| ADK ELISA Kit | publications with ADA and ADK |
| NT5E ELISA Kit | publications with ADA and NT5E |
Pathways associated with ADA ELISA Kit
| C-MYB Transcription Factor Network Pathway | C-MYB Transcription Factor Network Pathway |
| Metabolic Pathways | Metabolic Pathways |
| Metabolism Pathway | Metabolism Pathway |
| Metabolism Of Nucleotides Pathway | Metabolism Of Nucleotides Pathway |
| Primary Immunodeficiency Pathway | Primary Immunodeficiency Pathway |
| Purine Metabolism Pathway | Purine Metabolism Pathway |
| Purine Salvage Pathway | Purine Salvage Pathway |
Diseases associated with ADA ELISA Kit
| Nervous System Diseases | publications with ADA and Nervous System Diseases |
| Brain Diseases | publications with ADA and Brain Diseases |
| Heart Diseases | publications with ADA and Heart Diseases |
| Inflammation | publications with ADA and Inflammation |
| Necrosis | publications with ADA and Necrosis |
| Hypertension | publications with ADA and Hypertension |
| Disease Models, Animal | publications with ADA and Disease Models, Animal |
| Pain | publications with ADA and Pain |
| Liver Diseases | publications with ADA and Liver Diseases |
| Kidney Diseases | publications with ADA and Kidney Diseases |
Organs/Tissues associated with ADA ELISA Kit
| Blood | publications with ADA and Blood |
| Bone | publications with ADA and Bone |
| Heart | publications with ADA and Heart |
| Brain | publications with ADA and Brain |
| Bone Marrow | publications with ADA and Bone Marrow |
| Liver | publications with ADA and Liver |
| Lung | publications with ADA and Lung |
| Spleen | publications with ADA and Spleen |
| Kidney | publications with ADA and Kidney |
| Thymus | publications with ADA and Thymus |
