KEY FEATURES
| Full Name | Adenylate Cyclase 9 |
|---|---|
| Synonym | AC9; ATP pyrophosphate-lyase 9; Adenylate cyclase type IX |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.13ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ADCY9 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADCY9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADCY9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADCY9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADCY9. You can calculate the concentration of Human ADCY9 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ADCY9 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.95 | 3.07 | 7.99 | 0.89 | 2.95 | 8.79 |
| Standard deviation | 0.05 | 0.18 | 0.27 | 0.05 | 0.14 | 0.33 |
| CV(%) | 5.52 | 5.9 | 3.44 | 5.61 | 4.75 | 3.71 |
RECOVERY
The recovery of Human ADCY9 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 95-108 | 102 |
| EDTA plasma (n=8) | 85-99 | 92 |
| Cell culture media (n=8) | 90-104 | 95 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ADCY9 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with ADCY9 ELISA Kits
| ADCY6 ELISA Kit | publications with ADCY9 and ADCY6 |
| ADCY8 ELISA Kit | publications with ADCY9 and ADCY8 |
| ADRB2 ELISA Kit | publications with ADCY9 and ADRB2 |
| PDE4D ELISA Kit | publications with ADCY9 and PDE4D |
| PDE8A ELISA Kit | publications with ADCY9 and PDE8A |
| PDE4A ELISA Kit | publications with ADCY9 and PDE4A |
Pathways associated with ADCY9 ELISA Kit
| Activation Of GABAB Receptors Pathway | Activation Of GABAB Receptors Pathway |
| Adenylate Cyclase Activating Pathway | Adenylate Cyclase Activating Pathway |
| Adenylate Cyclase Inhibitory Pathway | Adenylate Cyclase Inhibitory Pathway |
| Adrenergic Signaling In Cardiomyocytes Pathway | Adrenergic Signaling In Cardiomyocytes Pathway |
| Aquaporin-mediated Transport Pathway | Aquaporin-mediated Transport Pathway |
| Bile Secretion Pathway | Bile Secretion Pathway |
| Ca-dependent Events Pathway | Ca-dependent Events Pathway |
| CaM Pathway | CaM Pathway |
Diseases associated with ADCY9 ELISA Kit
| Nervous System Diseases | publications with ADCY9 and Nervous System Diseases |
| Brain Diseases | publications with ADCY9 and Brain Diseases |
| Neurobehavioral Manifestations | publications with ADCY9 and Neurobehavioral Manifestations |
| Neoplasm Metastasis | publications with ADCY9 and Neoplasm Metastasis |
| Liver Neoplasms | publications with ADCY9 and Liver Neoplasms |
| Disease Models, Animal | publications with ADCY9 and Disease Models, Animal |
| Liver Neoplasms, Experimental | publications with ADCY9 and Liver Neoplasms, Experimental |
| Depressive Disorder | publications with ADCY9 and Depressive Disorder |
| Cardiovascular Diseases | publications with ADCY9 and Cardiovascular Diseases |
| Liver Diseases | publications with ADCY9 and Liver Diseases |
Organs/Tissues associated with ADCY9 ELISA Kit
| Brain | publications with ADCY9 and Brain |
| Nerve | publications with ADCY9 and Nerve |
| Prostate | publications with ADCY9 and Prostate |
| Testis | publications with ADCY9 and Testis |
| Kidney | publications with ADCY9 and Kidney |
| Eye | publications with ADCY9 and Eye |
| Liver | publications with ADCY9 and Liver |
| Ovary | publications with ADCY9 and Ovary |
| Muscle | publications with ADCY9 and Muscle |
| Lung | publications with ADCY9 and Lung |
