KEY FEATURES
| Full Name | Arachidonate-15-Lipoxygenase |
|---|---|
| Synonym | LOX15; 15-LOX2; 15-LOX1; 15-LOX; 12-LOX; ALOX15; 15-LO; 15-LPG; LOG15; Lipoxygenase 15; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate omega-6 lipoxygenase |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 1.56-100ng/mL |
| Sensitivity | 0.55ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ALOX15 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ALOX15. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALOX15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALOX15, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ALOX15. You can calculate the concentration of Human ALOX15 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ALOX15 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 4.69 | 8.45 | 49.45 | 4.76 | 9.16 | 53.58 |
| Standard deviation | 0.28 | 0.35 | 1.65 | 0.3 | 0.37 | 1.69 |
| CV(%) | 5.9 | 4.14 | 3.34 | 6.4 | 4.07 | 3.15 |
RECOVERY
The recovery of Human ALOX15 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-98 | 93 |
| EDTA plasma (n=8) | 92-107 | 98 |
| Cell culture media (n=8) | 92-108 | 99 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ALOX15 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with 15-LO ELISA Kits
| PTGS2 ELISA Kit | publications with 15-LO and PTGS2 |
| PTGS1 ELISA Kit | publications with 15-LO and PTGS1 |
| PLA2G4A ELISA Kit | publications with 15-LO and PLA2G4A |
| STAT6 ELISA Kit | publications with 15-LO and STAT6 |
| ALOX5AP ELISA Kit | publications with 15-LO and ALOX5AP |
| GPX3 ELISA Kit | publications with 15-LO and GPX3 |
| PLA2G5 ELISA Kit | publications with 15-LO and PLA2G5 |
| LTC4S ELISA Kit | publications with 15-LO and LTC4S |
Pathways associated with 15-LO ELISA Kit
| Arachidonic Acid Metabolism Pathway | Arachidonic Acid Metabolism Pathway |
| Eicosanoid Synthesis Pathway | Eicosanoid Synthesis Pathway |
| IL4-mediated Signaling Events Pathway | IL4-mediated Signaling Events Pathway |
| Linoleic Acid Metabolism Pathway | Linoleic Acid Metabolism Pathway |
| Metabolic Pathways | Metabolic Pathways |
| Metabolism Pathway | Metabolism Pathway |
| Metabolism Of Lipids And Lipoproteins Pathway | Metabolism Of Lipids And Lipoproteins Pathway |
Diseases associated with 15-LO ELISA Kit
| Inflammation | publications with 15-LO and Inflammation |
| Cardiovascular Diseases | publications with 15-LO and Cardiovascular Diseases |
| Atherosclerosis | publications with 15-LO and Atherosclerosis |
| Disease Models, Animal | publications with 15-LO and Disease Models, Animal |
| Lung Diseases | publications with 15-LO and Lung Diseases |
| Hypertension | publications with 15-LO and Hypertension |
| Necrosis | publications with 15-LO and Necrosis |
| Kidney Diseases | publications with 15-LO and Kidney Diseases |
| Heart Diseases | publications with 15-LO and Heart Diseases |
| Fibrosis | publications with 15-LO and Fibrosis |
Organs/Tissues associated with 15-LO ELISA Kit
| Lung | publications with 15-LO and Lung |
| Prostate | publications with 15-LO and Prostate |
| Trachea | publications with 15-LO and Trachea |
| Intestine | publications with 15-LO and Intestine |
| Adipose Tissue | publications with 15-LO and Adipose Tissue |
| Embryonic Tissue | publications with 15-LO and Embryonic Tissue |
| Placenta | publications with 15-LO and Placenta |
| Lymph Node | publications with 15-LO and Lymph Node |
| Testis | publications with 15-LO and Testis |
| Mouth | publications with 15-LO and Mouth |
