KEY FEATURES
| Full Name | Apolipoprotein B100 |
|---|---|
| Synonym | Apo-B100 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 93.75-6000ng/mL |
| Sensitivity | 44.84ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human APOB100 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human APOB100. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human APOB100 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human APOB100, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human APOB100. You can calculate the concentration of Human APOB100 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human APOB100 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 302.32 | 927.4 | 2963.98 | 293.81 | 974.83 | 3172.1 |
| Standard deviation | 15.6 | 53.05 | 89.51 | 18.36 | 55.66 | 110.07 |
| CV(%) | 5.16 | 5.72 | 3.02 | 6.25 | 5.71 | 3.47 |
RECOVERY
The recovery of Human APOB100 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-104 | 98 |
| EDTA plasma (n=8) | 95-107 | 101 |
| Cell culture media (n=8) | 87-102 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human APOB100 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Pathways associated with ELISA Kit
| Binding And Uptake Of Ligands By Scavenger Receptors Pathway | Binding And Uptake Of Ligands By Scavenger Receptors Pathway |
| Cell Surface Interactions At The Vascular Wall Pathway | Cell Surface Interactions At The Vascular Wall Pathway |
| Chylomicron-mediated Lipid Transport Pathway | Chylomicron-mediated Lipid Transport Pathway |
| Disease Pathway | Disease Pathway |
| Diseases Associated With Visual Transduction Pathway | Diseases Associated With Visual Transduction Pathway |
| FOXA1 Transcription Factor Network Pathway | FOXA1 Transcription Factor Network Pathway |
| Fat Digestion And Absorption Pathway | Fat Digestion And Absorption Pathway |
| Hemostasis Pathway | Hemostasis Pathway |
| LDL-mediated Lipid Transport Pathway | LDL-mediated Lipid Transport Pathway |
Diseases associated with APOB100 ELISA Kit
| Cardiovascular Diseases | publications with APOB100 and Cardiovascular Diseases |
| Dyslipidemias | publications with APOB100 and Dyslipidemias |
| Atherosclerosis | publications with APOB100 and Atherosclerosis |
| Hyperlipidemias | publications with APOB100 and Hyperlipidemias |
| Coronary Disease | publications with APOB100 and Coronary Disease |
| Heart Diseases | publications with APOB100 and Heart Diseases |
| Hypercholesterolemia | publications with APOB100 and Hypercholesterolemia |
| Hyperlipoproteinemia Type II | publications with APOB100 and Hyperlipoproteinemia Type II |
| Insulin Resistance | publications with APOB100 and Insulin Resistance |
| Hypertriglyceridemia | publications with APOB100 and Hypertriglyceridemia |
Organs/Tissues associated with APOB100 ELISA Kit
| Blood | publications with APOB100 and Blood |
| Liver | publications with APOB100 and Liver |
| Heart | publications with APOB100 and Heart |
| Intestine | publications with APOB100 and Intestine |
| Kidney | publications with APOB100 and Kidney |
| Muscle | publications with APOB100 and Muscle |
| Brain | publications with APOB100 and Brain |
| Lung | publications with APOB100 and Lung |
| Embryonic Tissue | publications with APOB100 and Embryonic Tissue |
| Spleen | publications with APOB100 and Spleen |
